Application of different types of CRISPR/Cas-based systems in bacteria
Z Liu, H Dong, Y Cui, L Cong, D Zhang - Microbial cell factories, 2020 - Springer
As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …
Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation
AA Dominguez, WA Lim, LS Qi - Nature reviews Molecular cell biology, 2016 - nature.com
The bacterial CRISPR–Cas9 system has emerged as a multifunctional platform for
sequence-specific regulation of gene expression. This Review describes the development of …
sequence-specific regulation of gene expression. This Review describes the development of …
Spatiotemporal control of CRISPR/Cas9 gene editing
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein
9 (CRISPR/Cas9) gene editing technology, as a revolutionary breakthrough in genetic …
9 (CRISPR/Cas9) gene editing technology, as a revolutionary breakthrough in genetic …
Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi
The vast majority of bacteria, including human pathogens and microbiome species, lack
genetic tools needed to systematically associate genes with phenotypes. This is the major …
genetic tools needed to systematically associate genes with phenotypes. This is the major …
CRISPR technologies for bacterial systems: current achievements and future directions
Throughout the decades of its history, the advances in bacteria-based bio-industries have
coincided with great leaps in strain engineering technologies. Recently unveiled clustered …
coincided with great leaps in strain engineering technologies. Recently unveiled clustered …
Next generation prokaryotic engineering: the CRISPR-Cas toolkit
The increasing demand for environmentally friendly production processes of green
chemicals and fuels has stimulated research in microbial metabolic engineering. CRISPR …
chemicals and fuels has stimulated research in microbial metabolic engineering. CRISPR …
CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms
Genetic manipulation of microorganisms has been crucial in understanding their biology, yet
for many microbial species, robust tools for comprehensive genetic analysis were lacking …
for many microbial species, robust tools for comprehensive genetic analysis were lacking …
[HTML][HTML] Genetic and epigenetic control of gene expression by CRISPR–Cas systems
A Lo, L Qi - F1000Research, 2017 - ncbi.nlm.nih.gov
The discovery and adaption of bacterial clustered regularly interspaced short palindromic
repeats (CRISPR)–CRISPR-associated (Cas) systems has revolutionized the way …
repeats (CRISPR)–CRISPR-associated (Cas) systems has revolutionized the way …
Targeted-antibacterial-plasmids (TAPs) combining conjugation and CRISPR/Cas systems achieve strain-specific antibacterial activity
A Reuter, C Hilpert, A Dedieu-Berne… - Nucleic acids …, 2021 - academic.oup.com
The global emergence of drug-resistant bacteria leads to the loss of efficacy of our
antibiotics arsenal and severely limits the success of currently available treatments. Here …
antibiotics arsenal and severely limits the success of currently available treatments. Here …
Bacterial CRISPR: accomplishments and prospects
Highlights•Cas9 provides a powerful counter-selection tool for genome editing in
bacteria.•CRISPRi is a versatile method for repressing gene expression.•With further …
bacteria.•CRISPRi is a versatile method for repressing gene expression.•With further …