Recent advances in genome editing technologies have magnified the prospect of single-
dose cures for many genetic diseases. For most genetic disorders, precise DNA correction is …

TALEN were the first easy-to-use genome editing technology and sparked the genome
editing revolution. Their application in multiple species brought targeted mutagenesis to the …

Bacterial toxin DddA-derived cytosine base editors (DdCBEs)—composed of split DddAtox
(a cytosine deaminase specific to double-stranded DNA), custom-designed TALE …

Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

Prion disease is caused by misfolding of the prion protein (PrP) into pathogenic self-
propagating conformations, leading to rapid-onset dementia and death. However …

Recent progress in developing programmable nucleases, such as zinc-finger nucleases,
transcription activator–like effector nucleases (TALENs) and clustered regularly interspaced …

Background Genome of an organism has always fascinated life scientists. With the discovery
of restriction endonucleases, scientists were able to make targeted manipulations …

Genome editing with targeted nucleases and DNA donor templates homologous to the
break site has proven challenging in human hematopoietic stem and progenitor cells …

Genome or gene editing includes several new techniques to help scientists precisely modify
genome sequences. The techniques also enables us to alter the regulation of gene …

One of the most powerful strategies to investigate biology we have as scientists, is the ability
to transfer genetic material in a controlled and deliberate manner between organisms. When …