[HTML][HTML] Initiation of mRNA translation in bacteria: structural and dynamic aspects

CO Gualerzi, CL Pon - Cellular and Molecular Life Sciences, 2015 - Springer
Initiation of mRNA translation is a major checkpoint for regulating level and fidelity of protein
synthesis. Being rate limiting in protein synthesis, translation initiation also represents the …

Mechanism of protein biosynthesis in mammalian mitochondria

BE Christian, LL Spremulli - Biochimica et Biophysica Acta (BBA)-Gene …, 2012 - Elsevier
Protein synthesis in mammalian mitochondria produces 13 proteins that are essential
subunits of the oxidative phosphorylation complexes. This review provides a detailed outline …

The universally conserved prokaryotic GTPases

N Verstraeten, M Fauvart, W Versées… - … and Molecular Biology …, 2011 - Am Soc Microbiol
Members of the large superclass of P-loop GTPases share a core domain with a conserved
three-dimensional structure. In eukaryotes, these proteins are implicated in various crucial …

Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor

A Simonetti, S Marzi, IML Billas, A Tsai… - Proceedings of the …, 2013 - National Acad Sciences
Translation initiation factor 2 (IF2) promotes 30S initiation complex (IC) formation and 50S
subunit joining, which produces the 70S IC. The architecture of full-length IF2, determined by …

Insertion domain within mammalian mitochondrial translation initiation factor 2 serves the role of eubacterial initiation factor 1

AS Yassin, ME Haque, PP Datta… - Proceedings of the …, 2011 - National Acad Sciences
Mitochondria have their own translational machineries for the synthesis of thirteen
polypeptide chains that are components of the complexes that participate in the process of …

Translation initiation factor IF2 contributes to ribosome assembly and maturation during cold adaptation

A Brandi, L Piersimoni, NA Feto, R Spurio… - Nucleic Acids …, 2019 - academic.oup.com
Cold-stress in Escherichia coli induces de novo synthesis of translation initiation factors IF1,
IF2 and IF3 while ribosome synthesis and assembly slow down. Consequently, the …

Complementary charge-based interaction between the ribosomal-stalk protein L7/12 and IF2 is the key to rapid subunit association

X Ge, CS Mandava, C Lind, J Åqvist… - Proceedings of the …, 2018 - National Acad Sciences
The interaction between the ribosomal-stalk protein L7/12 (L12) and initiation factor 2 (IF2) is
essential for rapid subunit association, but the underlying mechanism is unknown. Here, we …

Initiation factor 2 crystal structure reveals a different domain organization from eukaryotic initiation factor 5B and mechanism among translational GTPases

D Eiler, J Lin, A Simonetti… - Proceedings of the …, 2013 - National Acad Sciences
The initiation of protein synthesis uses initiation factor 2 (IF2) in prokaryotes and a related
protein named eukaryotic initiation factor 5B (eIF5B) in eukaryotes. IF2 is a GTPase that …

Activation of initiation factor 2 by ligands and mutations for rapid docking of ribosomal subunits

MY Pavlov, A Zorzet, DI Andersson… - The EMBO …, 2011 - embopress.org
We previously identified mutations in the GTPase initiation factor 2 (IF2), located outside its
tRNA‐binding domain, compensating strongly (A‐type) or weakly (B‐type) for initiator tRNA …

[HTML][HTML] Structural dynamics of bacterial translation initiation factor IF2

H Wienk, E Tishchenko, R Belardinelli… - Journal of Biological …, 2012 - ASBMB
Bacterial translation initiation factor IF2 promotes ribosomal subunit association, recruitment,
and binding of fMet-tRNA to the ribosomal P-site and initiation dipeptide formation. Here, we …