Isothermal titration calorimetry (ITC) is a biophysical technique for measuring the formation
and dissociation of molecular complexes and has become an invaluable tool in many …

Incorporation of metallocofactors essential for the activity of many enyzmes is a major
mechanism of posttranslational modification. The cellular machinery required for these …

Cobalt is an essential trace element in both prokaryotes and eukaryotes. Nevertheless, it
occurs less frequently in metalloproteins than other transition metals. This low occurrence …

How cells ensure correct metallation of a given protein and whether a degree of promiscuity
in metal binding has evolved are largely unanswered questions. In a classic case, iron-and …

The review is devoted to biocatalysts based on actinobacteria of the genus Rhodococcus,
which are promising for environmental biotechnologies. In the review, biotechnological …

Neonicotinoid insecticide pollution in soil and water poses serious environmental risks.
Microbial biodegradation is an important neonicotinoid insecticide degradation pathway in …

The regions and types suitable mutations for bridging salt-bridges to intensify enzyme
stability are identified in this study. Using nitrile hydratase (NHase) as the model enzyme …

Although metallocofactors are ubiquitous in enzyme catalysis, how metal binding specificity
arises remains poorly understood, especially in the case of metals with similar primary …

Rhodococcus is a genus of mycolic-acid-containing actinomycetes that utilize a remarkable
variety of organic compounds as growth substrates. This degradation helps maintain the …

Acetamiprid is a neonicotinoid insecticide used worldwide that has caused environmental
pollution and adverse effects on ecosystems. Here, a novel bacterium, Pseudaminobacter …