An overview on enrichment methods for cell surface proteome profiling

Y Li, H Qin, M Ye - Journal of separation science, 2020 - Wiley Online Library
Cell surface proteins are essential for many important biological processes, including cell–
cell interactions, signal transduction, and molecular transportation. With the characteristics of …

Structural mass spectrometry of membrane proteins

AS Dafun, J Marcoux - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2022 - Elsevier
The analysis of proteins and protein complexes by mass spectrometry (MS) has come a long
way since the invention of electrospray ionization (ESI) in the mid 80s. Originally used to …

UniTmp: unified resources for transmembrane proteins

L Dobson, C Gerdán, S Tusnády… - Nucleic Acids …, 2024 - academic.oup.com
Abstract The UNIfied database of TransMembrane Proteins (UniTmp) is a comprehensive
and freely accessible resource of transmembrane protein structural information at different …

Sensitive profiling of cell surface proteome by using an optimized biotinylation method

Y Li, Y Wang, J Mao, Y Yao, K Wang, Q Qiao, Z Fang… - Journal of …, 2019 - Elsevier
Cell surface proteins are responsible for many critical functions. Systematical profiling of
these proteins would provide a unique molecular fingerprint to classify cells and provide …

Comprehensive discovery of the accessible primary amino group-containing segments from cell surface proteins by fine-tuning a high-throughput biotinylation method

T Langó, K Kuffa, G Tóth, L Turiák, L Drahos… - International Journal of …, 2022 - mdpi.com
Cell surface proteins, including transmembrane and other surface-anchored proteins, play a
key role in several critical cellular processes and have a strong diagnostic value. The …

LeishMANIAdb: a comparative resource for Leishmania proteins

GE Tusnády, A Zeke, ZE Kálmán, M Fatoux… - Database, 2023 - academic.oup.com
Leishmaniasis is a detrimental disease causing serious changes in quality of life and some
forms can lead to death. The disease is spread by the parasite Leishmania transmitted by …

CIRFESS: an interactive resource for querying the set of theoretically detectable peptides for cell surface and extracellular enrichment proteomic studies

M Waas, J Littrell, RL Gundry - Journal of the American Society for …, 2020 - ACS Publications
Cell surface transmembrane, extracellular, and secreted proteins are high value targets for
immunophenotyping, drug development, and studies related to intercellular communication …

Covalently modified carboxyl side chains on cell surface leads to a novel method toward topology analysis of transmembrane proteins

A Müller, T Langó, L Turiák, A Ács, G Várady… - Scientific Reports, 2019 - nature.com
The research on transmembrane proteins (TMPs) is quite widespread due to their biological
importance. Unfortunately, only a little amount of structural data is available of TMPs. Since …

APEX, a master key to resolve membrane topology in live cells

CM Yoo, HW Rhee - Biochemistry, 2019 - ACS Publications
Determining the topology of the membrane proteome is fundamental for understanding its
function at the membrane. However, conventional methods involving test tube reactions …

PolarProtPred: predicting apical and basolateral localization of transmembrane proteins using putative short linear motifs and deep learning

L Dobson, A Zeke, GE Tusnády - Bioinformatics, 2021 - academic.oup.com
Motivation Cell polarity refers to the asymmetric organization of cellular components in
various cells. Epithelial cells are the best-known examples of polarized cells, featuring …