Stimuli‐responsive peptides and proteins are an exciting class of smart biomaterials for
various applications and have received significant attention over the past decades. A wide …

Tag-assisted protein purification is a method of choice for both academic researches and
large-scale industrial demands. Application of the purification tags in the protein production …

Since its early development and use to decipher the genetic code, in vitro or 'cell-
free'systems have been used as an important research tool to understand biochemical …

For the first time, this work reported the one-step purification and targeted immobilization
process of a β-galactosidase (Gal) with the Cellulose Binding Domain (CBD) tag, by binding …

Enzyme self-assembly is a technology in which enzyme units can aggregate into ordered
macromolecules, assisted by scaffolds. In metabolic engineering, self-assembly strategies …

Downstream processing is considered one of the most challenging phases of industrial
manufacturing of therapeutic proteins, accounting for a large portion of the total production …

Self-cleaving affinity tags, based on engineered intein protein domains, have been touted as
a universal single step purification platform for tagless non-mAb proteins. These approaches …

Various methods have been used in targeted gene knock-in applications. CRISPR-based
knock-in strategies based on homology-independent repair pathways such as CRISPR HITI …

In this work, we describe a novel self‐cleaving affinity tag technology based on a highly
modified split‐intein cleaving element. In this system, which has recently been …

The in vitro biosynthesis of high-value compounds has become popular and attractive. The
convenient and simple strategy of enzyme immobilization has been significant for …