Electrochemical biosensors based on layer‐by‐layer assemblies

W Zhao, JJ Xu, HY Chen - Electroanalysis: An International …, 2006 - Wiley Online Library
Abstract Layer‐by‐layer (LBL) assemblies, which have undergone great progress in the past
decades, have been used widely in the construction of electrochemical biosensors. The LBL …

Protein crosslinking: uses in chemistry, biology and biotechnology

MN Gupta, M Perwez, M Sardar - Biocatalysis and …, 2020 - Taylor & Francis
Protein crosslinking is a part of many biological processes and is also carried out in vitro
under several controllable conditions with the help of any of the commercially available …

Purification and Characterization of Enzymes Exhibiting β-d-Xylosidase Activities in Stem Tissues of Arabidopsis

Z Minic, C Rihouey, CT Do, P Lerouge… - Plant …, 2004 - academic.oup.com
This work describes the purification and characterization of enzymes that exhibit β-d-
xylosidase activity in stem tissues of Arabidopsis. This is the first detailed investigation that …

Bioaffinity based immobilization of enzymes

M Saleemuddin - Thermal biosensors, bioactivity, bioaffinitty, 1999 - Springer
Procedures that utilize the affinities of biomolecules and ligands for the immobilization of
enzymes are gaining increasing acceptance in the construction of sensitive enzyme-based …

Layer-by-layer deposited nano-and micro-assemblies for insulin delivery: A review

K Yoshida, Y Hasebe, S Takahashi, K Sato… - Materials Science and …, 2014 - Elsevier
We present an overview of the recent progress in the development of layer-by-layer (LbL)
assembled thin films and microcapsules for insulin delivery. The LbL deposition of insulin …

Layer-by-layer construction of protein architectures through avidin–biotin and lectin–sugar interactions for biosensor applications

S Takahashi, K Sato, J Anzai - Analytical and bioanalytical chemistry, 2012 - Springer
In this review, the preparation and properties of protein architectures constructed by layer-by-
layer (LbL) deposition through avidin–biotin and concanavalin A (Con A)–sugar interactions …

Treatment of phenolic wastewater by horseradish peroxidase immobilized by bioaffinity layering

S Dalal, MN Gupta - Chemosphere, 2007 - Elsevier
Horseradish peroxidase was immobilized by bioaffinity layering and used for the treatment
of wastewater containing p-chlorophenol. For this purpose, lectin Concanavalin A was …

Versatile method of cholinesterase immobilisation via affinity bonds using Concanavalin A applied to the construction of a screen-printed biosensor

B Bucur, AF Danet, JL Marty - Biosensors and bioelectronics, 2004 - Elsevier
Development of new and more reliable methods to immobilise biomolecules has emerged
rapidly due to a continuous need for more stable, sensitive and reliable biosensors. This …

Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains

F Lisdat, R Dronov, H Möhwald, FW Scheller… - Chemical …, 2009 - pubs.rsc.org
The layer-by-layer adsorption technique based on the consecutive deposition of oppositely
charged species is suitable for the preparation of protein multilayers with fully electro-active …

Superbeads: Immobilization in “sweet” chemistry

J Nahalka, Z Liu, X Chen… - Chemistry–A European …, 2003 - Wiley Online Library
Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to
overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide …