Engineering synthetic biomolecular condensates

Y Dai, L You, A Chilkoti - Nature Reviews Bioengineering, 2023 - nature.com
The concept of phase-separation-mediated formation of biomolecular condensates provides
a new framework to understand cellular organization and cooperativity-dependent cellular …

Elastin-like polypeptides for biomedical applications

AK Varanko, JC Su, A Chilkoti - Annual review of biomedical …, 2020 - annualreviews.org
Elastin-like polypeptides (ELPs) are stimulus-responsive biopolymers derived from human
elastin. Their unique properties—including lower critical solution temperature phase …

Elastin‐like polypeptides: biomedical applications of tunable biopolymers

SR MacEwan, A Chilkoti - Peptide Science: Original Research …, 2010 - Wiley Online Library
Artificial repetitive polypeptides have grown in popularity as a bioinspired alternative to
synthetic polymers. The genetically encoded synthesis, monodispersity, potential lack of …

Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes

JR McDaniel, JA MacKay, FG Quiroz… - …, 2010 - ACS Publications
This paper reports a new strategy, recursive directional ligation by plasmid reconstruction
(PRe-RDL), to rapidly clone highly repetitive polypeptides of any sequence and specified …

Elastin-like polypeptides: Therapeutic applications for an emerging class of nanomedicines

J Despanie, JP Dhandhukia, SF Hamm-Alvarez… - Journal of Controlled …, 2016 - Elsevier
Elastin-like polypeptides (ELPs) constitute a genetically engineered class of 'protein
polymers' derived from human tropoelastin. They exhibit a reversible phase separation …

Design of Stimuli‐Responsive Peptides and Proteins

Y Li, G Yang, L Gerstweiler, SH Thang… - Advanced Functional …, 2023 - Wiley Online Library
Stimuli‐responsive peptides and proteins are an exciting class of smart biomaterials for
various applications and have received significant attention over the past decades. A wide …

Elastin‐like polypeptides as a purification tag for recombinant proteins

W Hassouneh, T Christensen… - Current protocols in …, 2010 - Wiley Online Library
This unit presents a recombinant protein purification method that employs an elastin‐like
polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase …

Natural and synthetic polymers as drug carriers for delivery of therapeutic proteins

MS Hamid Akash, K Rehman, S Chen - Polymer Reviews, 2015 - Taylor & Francis
In order to cure and treat health-related disorders, therapeutic substance must reach its
target site with a constant concentration over a long period of time. As oral administration is …

Translatable high drug loading drug delivery systems based on biocompatible polymer nanocarriers

W Chen, S Zhou, L Ge, W Wu, X Jiang - Biomacromolecules, 2018 - ACS Publications
Most nanocarriers possess low drug loading, resulting in frequently repeated administration
and thereby high cost and increased side effects. Furthermore, the characteristics of …

Elastin-like polypeptides revolutionize recombinant protein expression and their biomedical application

DM Floss, K Schallau, S Rose-John, U Conrad… - Trends in …, 2010 - cell.com
Elastin-like polypeptides (ELPs) are highly biocompatible and exhibit a potentially highly
useful property: that of a thermally responsive reversible phase transition. These …