If fully stretched out, a typical bacterial chromosome would be nearly 1 mm long,
approximately 1,000 times the length of a cell. Not only must cells massively compact their …

Homologous recombination maintains genomic integrity by repairing broken chromosomes.
The broken chromosome is partially resected to produce single-stranded DNA (ssDNA) that …

Once described as mere “bags of enzymes,” bacterial cells are in fact highly organized, with
many macromolecules exhibiting nonuniform localization patterns. Yet the physical and …

Chromosomes of a broad range of species, from bacteria to mammals, are structured by
large topological domains whose precise functional roles and regulatory mechanisms …

Despite the fundamental importance of transcription, a comprehensive analysis of RNA
polymerase (RNAP) behavior and its role in the nucleoid organization in vivo is lacking …

How genomes are organized within cells and how the 3D architecture of a genome
influences cellular functions are significant questions in biology. A bacterial genomic DNA …

Visualization of living E. coli nucleoids, defined by HupA-mCherry, reveals a discrete,
dynamic helical ellipsoid. Three basic features emerge.(1) Nucleoid density coalesces into …

The bacterial chromosome must be compacted more than 1,000-fold to fit into the
compartment in which it resides. How it is condensed, organized and ultimately segregated …

Spatial organization is a hallmark of all living systems. Even bacteria, the smallest forms of
cellular life, display defined shapes and complex internal organization, showcasing a highly …

The widely conserved ParABS system plays a major role in bacterial chromosome
segregation. How the components of this system work together to generate translocation …