Exploiting chemical protein synthesis to study the role of tyrosine sulfation on anticoagulants from hematophagous organisms
JWC Maxwell, PME Hawkins, EE Watson… - Accounts of Chemical …, 2023 - ACS Publications
Conspectus Tyrosine sulfation is a post-translational modification (PTM) that modulates
function by mediating key protein–protein interactions. One of the early proteins shown to …
function by mediating key protein–protein interactions. One of the early proteins shown to …
Sulfotyrosine-mediated recognition of human thrombin by a tsetse fly anticoagulant mimics physiological substrates
BM Calisto, J Ripoll-Rozada, LJ Dowman, C Franck… - Cell Chemical …, 2021 - cell.com
Despite possessing only 32 residues, the tsetse thrombin inhibitor (TTI) is among the most
potent anticoagulants described, with sub-picomolar inhibitory activity against thrombin …
potent anticoagulants described, with sub-picomolar inhibitory activity against thrombin …
interferENZY: a web-based tool for enzymatic assay validation and standardized kinetic analysis
MF Pinto, A Baici, PJB Pereira… - Journal of molecular …, 2021 - Elsevier
Enzymatic assays are widely employed to characterize important allosteric and enzyme
modulation effects. The high sensitivity of these assays can represent a serious problem if …
modulation effects. The high sensitivity of these assays can represent a serious problem if …
Kinetic analysis misinterpretations due to the occurrence of enzyme inhibition by reaction product: Comparison between initial velocities and reaction time course …
Featured Application The integrated Michaelis–Menten equation (IMME) provides a viable
and accurate methodology for enzyme kinetic studies when the product is also an inhibitor …
and accurate methodology for enzyme kinetic studies when the product is also an inhibitor …
Estimation of initial rate from discontinuous progress data
PJ Halling - Biocatalysis and Biotransformation, 2020 - Taylor & Francis
When using discontinuous assay of reactions, initial rates are often estimated from a limited
number of time points. There has been no detailed study of how best to do this. In this work …
number of time points. There has been no detailed study of how best to do this. In this work …
Major improvements in robustness and efficiency during the screening of novel enzyme effectors by the 3-point kinetics assay
MF Pinto, F Figueiredo, A Silva… - … the Science of Drug …, 2021 - journals.sagepub.com
The throughput level currently reached by automatic liquid handling and assay monitoring
techniques is expected to facilitate the discovery of new modulators of enzyme activity …
techniques is expected to facilitate the discovery of new modulators of enzyme activity …