Glycan utilization systems in the human gut microbiota: a gold mine for structural discoveries

K Tamura, H Brumer - Current Opinion in Structural Biology, 2021 - Elsevier
Highlights•The human gut microbiome encodes an enormous number and variety of
enzymes, binding proteins, and transporters for utilization of complex glycans.•A plethora of …

Plant Protein O-Arabinosylation

BL Petersen, CA MacAlister, P Ulvskov - Frontiers in Plant Science, 2021 - frontiersin.org
A wide range of proteins with diverse functions in development, defense, and stress
responses are O-arabinosylated at hydroxyprolines (Hyps) within distinct amino acid motifs …

Two α-l-arabinofuranosidases from Bifidobacterium longum subsp. longum are involved in arabinoxylan utilization

M Komeno, Y Yoshihara, J Kawasaki… - Applied Microbiology …, 2022 - Springer
Arabinoxylan (AX) and arabinoxylooligosaccharides (AXOs) are carbohydrate sources
utilized by Bifidobacterium longum subsp. longum. However, their degradation pathways are …

Diversity and prevalence of Clostridium innocuum in the human gut microbiota

D Bhattacharjee, C Flores, C Woelfel-Monsivais… - Msphere, 2023 - Am Soc Microbiol
Clostridia are a polyphyletic group of Gram-positive, spore-forming anaerobes in the
Firmicutes phylum that significantly impact metabolism and functioning of the human …

Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes

S Fushinobu, M Abou Hachem - Biochemical Society …, 2021 - portlandpress.com
Bifidobacteria have attracted significant attention because they provide health-promoting
effects in the human gut. In this review, we present a current overview of the three …

Synthesis of naturally occurring β-l-arabinofuranosyl-l-arabinofuranoside structures towards the substrate specificity evaluation of β-l-arabinofuranosidase

A Ishiwata, K Fujita, S Fushinobu, K Tanaka… - Bioorganic & Medicinal …, 2022 - Elsevier
Abstract Methyl β-l-arabinofuranosyl-(1→ 2)-,-(1→ 3)-, and-(1→ 5)-α-l-arabinofuranosides
have been stereoselectively synthesized through 2-naphthylmethyl ether-mediated …

Novel 3-O-α-d-Galactosyl-α-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum

Y Sasaki, A Horigome, T Odamaki, JZ Xiao… - Applied and …, 2021 - Am Soc Microbiol
Gum arabic arabinogalactan (AG) protein (AGP) is a unique dietary fiber that is degraded
and assimilated by only specific strains of Bifidobacterium longum subsp. longum. Here, we …

Substrate complex structure, active site labeling and catalytic role of the zinc ion in cysteine glycosidase

S Maruyama, K Sawano, S Amaki, T Suzuki… - …, 2022 - academic.oup.com
Abstract β-l-Arabinofuranosidase HypBA1 from Bifidobacterium longum belongs to the
glycoside hydrolase family 127. At the active site of HypBA1, a cysteine residue (Cys417) …

Bifidobacterial GH146 β‐l‐Arabinofuranosidase (Bll4HypBA1) as the Last Enzyme for the Complete Removal of Oligoarabinofuranosides from Hydroxyproline‐Rich …

A Ishiwata, H Tsunomachi, K Kameyama… - …, 2023 - Wiley Online Library
In plant cell walls, the hydroxyproline‐rich glycoproteins (HRGPs) such as extensin contain
oligoarabinofuranoside linked to a hydroxyproline (Hyp) residue. The mature …

Role of β-L-Arabinofuranosidases in Intestinal Bacteria

K Fujita, A Ishiwata, S Fushinobu - Trends in Glycoscience and …, 2024 - jstage.jst.go.jp
Abstract β-L-Arabinofuranosidase (β-Arafase) is an enzyme belonging to the glycoside
hydrolase families GH127/137/142/146 that hydrolyze β-L-arabinofuranoside (Araf) from …