Understanding the rules of life is one of the most important scientific endeavours and has
revolutionised both biology and biotechnology. Remarkable advances in observation …

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at
high spatial and temporal resolution. However, the utility of existing GEVIs has been limited …

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record
brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet …

We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with
fast and complete maturation, as well as record brightness, quantum yield (75%) and …

The slow maturation time of fluorescent proteins (FPs) limits the temporal accuracy of
measurements of rapid processes such as gene expression dynamics and effectively …

We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a
tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum …

Many genetically encoded biosensors use Förster resonance energy transfer (FRET) to
dynamically report biomolecular activities. While pairs of cyan and yellow fluorescent …

Genetically encoded calcium indicators (GECIs) are powerful tools for systems
neuroscience. Here we describe red, single-wavelength GECIs,“RCaMPs,” engineered from …

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a
fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic …