Bacterial virus forcing of bacterial O-antigen shields: lessons from Coliphages

AV Letarov - International Journal of Molecular Sciences, 2023 - mdpi.com
In most Gram-negative bacteria, outer membrane (OM) lipopolysaccharide (LPS) molecules
carry long polysaccharide chains known as the O antigens or O polysaccharides (OPS). The …

Bacterial synthetic biology: tools for novel drug discovery

X Wang, N Zhou, B Wang - Expert Opinion on Drug Discovery, 2023 - Taylor & Francis
Introduction Bacterial synthetic biology has provided powerful tools to revolutionize the drug
discovery process. These tools can be harnessed to generate bacterial novel …

P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri

YW Huan, V Torraca, R Brown, J Fa-Arun… - ACS synthetic …, 2023 - ACS Publications
The discovery of clustered, regularly interspaced, short palindromic repeats (CRISPR) and
the Cas9 RNA-guided nuclease provides unprecedented opportunities to selectively kill …

Tail-engineered phage P2 enables delivery of antimicrobials into multiple gut pathogens

J Fa-Arun, YW Huan, E Darmon, B Wang - ACS Synthetic Biology, 2023 - ACS Publications
Bacteriophages can be reprogrammed to deliver antimicrobials for therapeutic and
biocontrol purposes and are a promising alternative treatment to antimicrobial-resistant …

Interaction of bacteriophage P1 with an epiphytic Pantoea agglomerans strain—the role of the interplay between various mobilome elements

K Giermasińska-Buczek, J Gawor… - Frontiers in …, 2024 - frontiersin.org
P1 is a model, temperate bacteriophage of the 94 kb genome. It can lysogenize
representatives of the Enterobacterales order. In lysogens, it is maintained as a plasmid. We …

An adenine/thymidine-rich region is integral to RepL-mediated DNA replication

YW Huan, R Brown, B Wang - Frontiers in Microbiology, 2023 - frontiersin.org
The lytic replication of bacteriophage P1 requires RepL expression and the lytic stage origin,
oriL, which is postulated to be located within repL gene sequence. The exact sequence of …

Establishing new technologies for the development of targeted virotherapies

T Cunliffe - 2023 - orca.cardiff.ac.uk
Despite improvements in cancer treatments, there is still an unmet clinical need for effective
therapeutic agents that are highly tumour specific. Oncolytic viruses hold many …