Advances in multiplexed techniques for the detection and quantification of microRNAs
MicroRNA detection is currently a crucial analytical chemistry challenge: almost 2000
papers were referenced in PubMed in 2018 and 2019 for the keywords “miRNA detection …
papers were referenced in PubMed in 2018 and 2019 for the keywords “miRNA detection …
Nucleic acid tests for clinical translation
Nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are
natural biopolymers composed of nucleotides that store, transmit, and express genetic …
natural biopolymers composed of nucleotides that store, transmit, and express genetic …
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs
The use of microRNAs as clinical cancer biomarkers is hindered by the absence of accurate,
fast and inexpensive assays for their detection in biofluids. Here we report a one-step and …
fast and inexpensive assays for their detection in biofluids. Here we report a one-step and …
Principles and applications of nucleic acid strand displacement reactions
Dynamic DNA nanotechnology, a subfield of DNA nanotechnology, is concerned with the
study and application of nucleic acid strand-displacement reactions. Strand-displacement …
study and application of nucleic acid strand-displacement reactions. Strand-displacement …
Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues
Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity
and throughput. Here we report immunostaining with signal amplification by exchange …
and throughput. Here we report immunostaining with signal amplification by exchange …
Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon
The in vivo optical imaging of RNA biomarkers of inflammation is hindered by low signal-to-
background ratios, owing to non-specific signal amplification in healthy tissues. Here we …
background ratios, owing to non-specific signal amplification in healthy tissues. Here we …
Third-generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust
HMT Choi, M Schwarzkopf, ME Fornace… - …, 2018 - journals.biologists.com
In situ hybridization based on the mechanism of the hybridization chain reaction (HCR) has
addressed multi-decade challenges that impeded imaging of mRNA expression in diverse …
addressed multi-decade challenges that impeded imaging of mRNA expression in diverse …
Expansion microscopy: principles and uses in biological research
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …
resolution. We recently discovered that preserved biological specimens can be physically …
Macrophages directly contribute collagen to scar formation during zebrafish heart regeneration and mouse heart repair
FC Simões, TJ Cahill, A Kenyon… - Nature …, 2020 - nature.com
Canonical roles for macrophages in mediating the fibrotic response after a heart attack
include extracellular matrix turnover and activation of cardiac fibroblasts to initiate collagen …
include extracellular matrix turnover and activation of cardiac fibroblasts to initiate collagen …
Isothermal amplification of nucleic acids: The race for the next “gold standard”
BB Oliveira, B Veigas, PV Baptista - Frontiers in Sensors, 2021 - frontiersin.org
Nucleic acid amplification technologies (NAATs) have become fundamental tools in
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …