Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

2. INTRODUCTION The investigation of many fundamental processes in life sciences
requires straightforward tools for fast, sensitive, reliable, and reproducible detection of …

Direct stochastic optical reconstruction microscopy (d STORM) uses conventional
fluorescent probes such as labeled antibodies or chemical tags for subdiffraction resolution …

Anyone who has used a light microscope has wished that its resolution could be a little
better. Now, after centuries of gradual improvements, fluorescence microscopy has made a …

Research on fluorescent semiconductor nanocrystals (also known as quantum dots or
qdots) has evolved over the past two decades from electronic materials science to biological …

Biological structures span many orders of magnitude in size, but far-field visible light
microscopy suffers from limited resolution. A new method for fluorescence imaging has been …

Achieving a spatial resolution that is not limited by the diffraction of light, recent
developments of super-resolution fluorescence microscopy techniques allow the …

Myosin V is a dimeric molecular motor that moves processively on actin, with the center of
mass moving∼ 37 nanometers for each adenosine triphosphate hydrolyzed. We have …

Dielectric microspheres with appropriate refractive index can image objects with super-
resolution, that is, with a precision well beyond the classical diffraction limit. A microsphere is …

For more than a century, the resolution of focusing light microscopy has been limited by
diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently …