The encoded biosynthesis of proteins provides the ultimate paradigm for high-fidelity
synthesis of long polymers of defined sequence and composition, but it is limited to …

Abstract “Recombineering,” in vivo genetic engineering with short DNA homologies, is
changing how constructs are made. The methods are simple, precise, efficient, rapid, and …

Klebsiella pneumoniae is an important cause of multidrug‐resistant infections worldwide.
Recent studies highlight the emergence of multidrug‐resistant K. pneumoniae strains which …

Etiological agents of acute, persistent, or relapsing clinical infections are often refractory to
antibiotics due to multidrug resistance and/or antibiotic tolerance. Pseudomonas aeruginosa …

High levels of antibiotic tolerance are a hallmark of bacterial biofilms. In contrast to well-
characterized inherited antibiotic resistance, molecular mechanisms leading to reversible …

The formation of biofilm results in a major lifestyle switch that is thought to affect the
expression of multiple genes and operons. We used DNA arrays to study the global effect of …

Random transposon mutagenesis is the strategy of choice for associating a phenotype with
its unknown genetic determinants. It is generally performed by mobilization of a conditionally …

Abstract Background The λ Red recombineering technology has been used extensively in
Escherichia coli and Salmonella typhimurium for easy PCR-mediated generation of deletion …

Background Klebsiella pneumoniae is an important gram-negative opportunistic pathogen
causing primarily urinary tract infections, respiratory infections, and bacteraemia. The ability …

Curli fibers could be described as a virulence factor able to confer adherence properties to
both abiotic and eukaryotic surfaces. The ability to adapt rapidly to changing environmental …