[HTML][HTML] Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
S Zhang, J Shen, D Li, Y Cheng - Theranostics, 2021 - ncbi.nlm.nih.gov
CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years,
however, the translation of this biotechnology into therapy has been hindered by efficient …
however, the translation of this biotechnology into therapy has been hindered by efficient …
DNA repair pathway choices in CRISPR-Cas9-mediated genome editing
Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage
JP Zhang, XL Li, GH Li, W Chen, C Arakaki… - Genome biology, 2017 - Springer
Background Precise genome editing via homology-directed repair (HDR) after double-
stranded DNA (dsDNA) cleavage facilitates functional genomic research and holds promise …
stranded DNA (dsDNA) cleavage facilitates functional genomic research and holds promise …
CRISPR/Cas9-induced targeted mutagenesis and gene replacement to generate long-shelf life tomato lines
Q Yu, B Wang, N Li, Y Tang, S Yang, T Yang, J Xu… - Scientific reports, 2017 - nature.com
Quickly and precisely gain genetically enhanced breeding elites with value-adding
performance traits is desired by the crop breeders all the time. The present of gene editing …
performance traits is desired by the crop breeders all the time. The present of gene editing …
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems
Programmable nucleases enable engineering of the genome by utilizing endogenous DNA
double-strand break (DSB) repair pathways. Although homologous recombination (HR) …
double-strand break (DSB) repair pathways. Although homologous recombination (HR) …
Homology-mediated end joining-based targeted integration using CRISPR/Cas9
X Yao, X Wang, X Hu, Z Liu, J Liu, H Zhou, X Shen… - Cell research, 2017 - nature.com
Targeted integration of transgenes can be achieved by strategies based on homologous
recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end …
recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end …
Making waves: New developments in toxicology with the zebrafish
KA Horzmann, JL Freeman - Toxicological Sciences, 2018 - academic.oup.com
The laboratory zebrafish (Danio rerio) is now an accepted model in toxicologic research.
The zebrafish model fills a niche between in vitro models and mammalian biomedical …
The zebrafish model fills a niche between in vitro models and mammalian biomedical …
Little fish, big data: zebrafish as a model for cardiovascular and metabolic disease
The burden of cardiovascular and metabolic diseases worldwide is staggering. The
emergence of systems approaches in biology promises new therapies, faster and cheaper …
emergence of systems approaches in biology promises new therapies, faster and cheaper …
Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair
Abstract CRISPR/Cas9-induced site-specific DNA double-strand breaks (DSBs) can be
repaired by homology-directed repair (HDR) or non-homologous end joining (NHEJ) …
repaired by homology-directed repair (HDR) or non-homologous end joining (NHEJ) …
Advancements in zebrafish applications for 21st century toxicology
GR Garcia, PD Noyes, RL Tanguay - Pharmacology & therapeutics, 2016 - Elsevier
The zebrafish model is the only available high-throughput vertebrate assessment system,
and it is uniquely suited for studies of in vivo cell biology. A sequenced and annotated …
and it is uniquely suited for studies of in vivo cell biology. A sequenced and annotated …