[HTML][HTML] Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%
J Goedhart, D Von Stetten, M Noirclerc-Savoye… - Nature …, 2012 - nature.com
Cyan variants of green fluorescent protein are widely used as donors in Förster resonance
energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent …
energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent …
[HTML][HTML] Current advances in synchrotron radiation instrumentation for MX experiments
Following pioneering work 40 years ago, synchrotron beamlines dedicated to
macromolecular crystallography (MX) have improved in almost every aspect as …
macromolecular crystallography (MX) have improved in almost every aspect as …
Crystal structure of a light-driven sodium pump
Recently, the first known light-driven sodium pumps, from the microbial rhodopsin family,
were discovered. We have solved the structure of one of them, Krokinobacter eikastus …
were discovered. We have solved the structure of one of them, Krokinobacter eikastus …
Advancements in macromolecular crystallography: From past to present
Protein Crystallography or Macromolecular Crystallography (MX) started as a new discipline
of science with the pioneering work on the determination of the protein crystal structures by …
of science with the pioneering work on the determination of the protein crystal structures by …
[HTML][HTML] Crystal structure of a common GPCR-binding interface for G protein and arrestin
M Szczepek, F Beyrière, KP Hofmann, M Elgeti… - Nature …, 2014 - nature.com
G-protein-coupled receptors (GPCRs) transmit extracellular signals to activate intracellular
heterotrimeric G proteins (Gαβγ) and arrestins. For G protein signalling, the Gα C-terminus …
heterotrimeric G proteins (Gαβγ) and arrestins. For G protein signalling, the Gα C-terminus …
Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein
A Royant, M Noirclerc-Savoye - Journal of structural biology, 2011 - Elsevier
Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent
Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase …
Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase …
[HTML][HTML] Snapshots of enzymatic Baeyer-Villiger catalysis: oxygen activation and intermediate stabilization
R Orru, HM Dudek, C Martinoli, DET Pazmiño… - Journal of Biological …, 2011 - ASBMB
Baeyer-Villiger monooxygenases catalyze the oxidation of carbonylic substrates to ester or
lactone products using NADPH as electron donor and molecular oxygen as oxidative …
lactone products using NADPH as electron donor and molecular oxygen as oxidative …
Intrinsic dynamics in ECFP and Cerulean control fluorescence quantum yield
M Lelimousin, M Noirclerc-Savoye… - Biochemistry, 2009 - ACS Publications
Enhanced cyan fluorescent protein (ECFP) and its variant Cerulean are genetically encoded
fluorophores widely used as donors in FRET-based cell imaging experiments. First, we have …
fluorophores widely used as donors in FRET-based cell imaging experiments. First, we have …
[HTML][HTML] In crystallo optical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF
D von Stetten, T Giraud, P Carpentier… - … Section D: Biological …, 2015 - scripts.iucr.org
The analysis of structural data obtained by X-ray crystallography benefits from information
obtained from complementary techniques, especially as applied to the crystals themselves …
obtained from complementary techniques, especially as applied to the crystals themselves …
Crystal structure of plant light‐harvesting complex shows the active, energy‐transmitting state
Plants dissipate excess excitation energy as heat by non‐photochemical quenching (NPQ).
NPQ has been thought to resemble in vitro aggregation quenching of the major antenna …
NPQ has been thought to resemble in vitro aggregation quenching of the major antenna …