Insights into glycosidase mechanisms have come from X-ray crystallographic studies on
complexes with substrate analogs and inhibitors, representing all the intermediate species …

The three-dimensional structure of glycosidases and of their complexes and the study of
transition-state mimics reveal structural details that correlate with mechanism. Of particular …

Aims This review is intended to update our previous discussion on the chemoenzymatic
synthesis of carbohydrates and glycoconjugates,(1) with new emphasis placed on …

In recent years, substantial advances have been made in the engineering of glycosidases
and glycosyltransferases for the synthesis and degradation of glycan structures. Key …

Oligosaccharide synthesis is becoming increasingly important to the pharmaceutical
industry because these biomolecules have potential application as therapeutics. Enzymatic …

A startling array of added anions have been observed to function as replacement catalytic
nucleophiles in mutant glycosidases, including formate, azide, fluoride and other halides …

Background: Oligosaccharide synthesis is becoming increasingly important to industry as
diverse therapeutic roles for these molecules are discovered. The chemical synthesis of …

Enzymatic synthesis of β-mannosides avoids both of these problems.[6] Successful transfer
of β-mannosyl residues to a variety of acceptor sugars has been achieved with retaining …

Reducing end xylose-releasing exooligoxylanase (Rex, EC 3.2. 1.156) is an inverting GH
that hydrolyzes xylooligosaccharides (≥ X 3) to release X 1 at their reducing end. The wild …

Recently, we reported a general assay for enzyme catalysis based on the yeast three-hybrid
assay, Chemical Complementation, which is intended to expand the range of chemical …