Digital PCR applications in the SARS-CoV-2/COVID-19 era: a roadmap for future outbreaks

R Nyaruaba, C Mwaliko, D Dobnik… - Clinical Microbiology …, 2022 - Am Soc Microbiol
The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global public health …

[HTML][HTML] Gastrointestinal symptoms and fecal shedding of SARS-CoV-2 RNA suggest prolonged gastrointestinal infection

A Natarajan, S Zlitni, EF Brooks, SE Vance, A Dahlen… - Med, 2022 - cell.com
Background COVID-19 manifests with respiratory, systemic, and gastrointestinal (GI)
symptoms. 1, SARS-CoV-2 RNA is detected in respiratory and fecal samples, and recent …

[HTML][HTML] Improving the assessment of ecosystem and wildlife health: microbiome as an early indicator

MP Ribas, M García-Ulloa, J Espunyes… - Current opinion in …, 2023 - Elsevier
Highlights•Microbiome can be an early warning indicator of ecosystem and wildlife
health.•Microbiome responds to anthropogenic disturbances and management …

Respiratory syncytial virus (RSV) RNA in wastewater settled solids reflects RSV clinical positivity rates

B Hughes, D Duong, BJ White… - … & Technology Letters, 2022 - ACS Publications
Wastewater-based epidemiology (WBE) uses concentrations of infectious agent targets in
wastewater to infer infection trends in the contributing community. To date, WBE has been …

Longitudinal and quantitative fecal shedding dynamics of SARS-CoV-2, pepper mild mottle virus, and crAssphage

PJ Arts, JD Kelly, CM Midgley, K Anglin, S Lu… - Msphere, 2023 - Am Soc Microbiol
Wastewater-based epidemiology (WBE) emerged during the coronavirus disease 2019
(COVID-19) pandemic as a scalable and broadly applicable method for community-level …

Recommendations for the use of metagenomics for routine monitoring of antibiotic resistance in wastewater and impacted aquatic environments

BC Davis, C Brown, S Gupta, J Calarco… - Critical Reviews in …, 2023 - Taylor & Francis
Shotgun metagenomic sequencing of the collective genomic information carried across
microbial communities is emerging as a powerful approach for monitoring antibiotic …

A novel tiled amplicon sequencing assay targeting the tomato brown rugose fruit virus (ToBRFV) genome reveals widespread distribution in municipal wastewater …

D Nash, I Ellmen, JJ Knapp, R Menon, AK Overton… - Viruses, 2024 - mdpi.com
Tomato Brown Rugose Fruit Virus (ToBRFV) is a plant pathogen that infects important
Solanaceae crop species and can dramatically reduce tomato crop yields. The ToBRFV has …

Reducing bias in microbiome research: Comparing methods from sample collection to sequencing

J Kool, L Tymchenko, SA Shetty, S Fuentes - Frontiers in Microbiology, 2023 - frontiersin.org
Background Microbiota profiles are strongly influenced by many technical aspects that
impact the ability of researchers to compare results. To investigate and identify potential …

Smart toilets for monitoring COVID-19 surges: passive diagnostics and public health

TJ Ge, CT Chan, BJ Lee, JC Liao, S Park - NPJ Digital Medicine, 2022 - nature.com
Continued COVID-19 surges have highlighted the need for widespread testing in addition to
vaccination for disease containment. SARS-COV-2 RNA can be found in faecal matter …

Highly Multiplexed Reverse-Transcription Loop-Mediated Isothermal Amplification and Nanopore Sequencing (LAMPore) for Wastewater-Based Surveillance

S Kang, P Choi, A Maile-Moskowitz, CL Brown… - ACS Es&t …, 2024 - ACS Publications
Wastewater-based surveillance (WBS) has gained attention as a strategy to monitor and
provide an early warning for disease outbreaks. Here, we applied an isothermal gene …