We present a whole-cell fully dynamical kinetic model (WCM) of JCVI-syn3A, a minimal cell
with a reduced genome of 493 genes that has retained few regulatory proteins or small …

Bacterial cells are widely used to produce value-added products due to their versatility, ease
of manipulation, and the abundance of genome engineering tools. However, the efficiency of …

Mesoplasma florum is an emerging model organism for systems and synthetic biology due
to its small genome (∼ 800 kb) and fast growth rate. While M. florum was isolated and first …

Mesoplasma florum, a fast‐growing near‐minimal organism, is a compelling model to
explore rational genome designs. Using sequence and structural homology, the set of …

Recently, we presented a whole-cell kinetic model of the genetically minimal bacterium JCVI-
syn3A that described the coupled metabolic and genetic information processes and …

JCVI-syn3A is a genetically minimal bacterial cell, consisting of 493 genes and only a single
543 kbp circular chromosome. Syn3A's genome and physical size are approximately one …

During steady‐state cell growth, individual enzymatic fluxes can be directly inferred from
growth rate by mass conservation, but the inverse problem remains unsolved. Perturbing the …

The construction of synthetic cells from lifeless ensembles of molecules is expected to
require integration of hundreds of genetically-encoded functions whose collective capacities …

All cells are encapsulated by a lipid membrane which facilitates the interaction between life
and its environment. How life exploits the diverse mixtures of lipids that dictate membrane …

Over the past few decades, there has been a significant interest in the study of essential
genes, which are crucial for the survival of an organism under specific environmental …