Site-directed mutagenesis.

P Carter - Biochemical Journal, 1986 - ncbi.nlm.nih.gov
The last few years has seen rapid advances in the solid phase synthesis of oligonucleotides
(reviewed by Itakura et al., 1984; Gait, 1984; Caruthers, 1985) which combined with the …

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

JS Huston, D Levinson… - Proceedings of the …, 1988 - National Acad Sciences
A biosynthetic antibody binding site, which incorporated the variable domains of anti-digoxin
monoclonal antibody 26-10 in a single polypeptide chain (Mr= 26,354), was produced in …

Calmodulin7: recent insights into emerging roles in plant development and stress

R Basu, S Dutta, A Pal, M Sengupta… - Plant Molecular …, 2021 - Springer
Key message Analyses of the function of Arabidopsis Calmodulin7 (CAM7) in concert with
multiple regulatory proteins involved in various signal transduction processes. Abstract …

Secretion of cyclolysin, the calmodulin‐sensitive adenylate cyclase‐haemolysin bifunctional protein of Bordetella pertussis.

P Glaser, H Sakamoto, J Bellalou, A Ullmann… - The EMBO …, 1988 - embopress.org
The calmodulin‐sensitive adenylate cyclase of Bordetella pertussis, a 45 kd secreted
protein, is synthesized as a 1706 amino acid precursor. We have shown that this precursor …

Repair of oxidized proteins: identification of a new methionine sulfoxide reductase

R Grimaud, B Ezraty, JK Mitchell, D Lafitte… - Journal of Biological …, 2001 - ASBMB
Oxidation of methionine residues to methionine sulfoxide can lead to inactivation of proteins.
Methionine sulfoxide reductase (MsrA) has been known for a long time, and its repairing …

Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons

A Gennaris, B Ezraty, C Henry, R Agrebi, A Vergnes… - Nature, 2015 - nature.com
The reactive species of oxygen and chlorine damage cellular components, potentially
leading to cell death. In proteins, the sulfur-containing amino acid methionine is converted to …

The calmodulin‐sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia col

P Glaser, D Ladant, O Sezer, F Pichot… - Molecular …, 1988 - Wiley Online Library
The adenylate cyclase toxin of the prokaryote Bordetella pertussis is stimulated by the
eukaryotic regulatory protein, calmodulin. A general strategy, using the adenylate‐cyclase …

[HTML][HTML] Transgenic tobacco expressing a foreign calmodulin gene shows an enhanced production of active oxygen species

SA Harding, SH Oh, DM Roberts - The EMBO Journal, 1997 - embopress.org
A strategy for elucidating specific molecular targets of calcium and calmodulin in plant
defense responses has been developed. We have used a dominant‐acting calmodulin …

A novel calmodulin‐binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings

E Perruc, M Charpenteau, BC Ramirez… - The Plant …, 2004 - Wiley Online Library
A clone for a novel A rabidopsis t haliana calmodulin (CaM)‐b inding p rotein of 25 kDa
(AtCaMBP25) has been isolated by using a radiolabelled CaM probe to screen a cDNA …

Calmodulin binding domains: characterization of a phosphorylation and calmodulin binding site from myosin light chain kinase

TJ Lukas, WH Burgess, FG Prendergast, W Lau… - Biochemistry, 1986 - ACS Publications
A protein kinase phosphorylation site in chicken gizzard myosin light chain kinase (MLCK)
has been identified, and a synthetic peptide analogue of this site has been shown to be a …