Site-directed mutagenesis.
P Carter - Biochemical Journal, 1986 - ncbi.nlm.nih.gov
The last few years has seen rapid advances in the solid phase synthesis of oligonucleotides
(reviewed by Itakura et al., 1984; Gait, 1984; Caruthers, 1985) which combined with the …
(reviewed by Itakura et al., 1984; Gait, 1984; Caruthers, 1985) which combined with the …
Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.
JS Huston, D Levinson… - Proceedings of the …, 1988 - National Acad Sciences
A biosynthetic antibody binding site, which incorporated the variable domains of anti-digoxin
monoclonal antibody 26-10 in a single polypeptide chain (Mr= 26,354), was produced in …
monoclonal antibody 26-10 in a single polypeptide chain (Mr= 26,354), was produced in …
Calmodulin7: recent insights into emerging roles in plant development and stress
Key message Analyses of the function of Arabidopsis Calmodulin7 (CAM7) in concert with
multiple regulatory proteins involved in various signal transduction processes. Abstract …
multiple regulatory proteins involved in various signal transduction processes. Abstract …
Secretion of cyclolysin, the calmodulin‐sensitive adenylate cyclase‐haemolysin bifunctional protein of Bordetella pertussis.
P Glaser, H Sakamoto, J Bellalou, A Ullmann… - The EMBO …, 1988 - embopress.org
The calmodulin‐sensitive adenylate cyclase of Bordetella pertussis, a 45 kd secreted
protein, is synthesized as a 1706 amino acid precursor. We have shown that this precursor …
protein, is synthesized as a 1706 amino acid precursor. We have shown that this precursor …
Repair of oxidized proteins: identification of a new methionine sulfoxide reductase
Oxidation of methionine residues to methionine sulfoxide can lead to inactivation of proteins.
Methionine sulfoxide reductase (MsrA) has been known for a long time, and its repairing …
Methionine sulfoxide reductase (MsrA) has been known for a long time, and its repairing …
Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons
A Gennaris, B Ezraty, C Henry, R Agrebi, A Vergnes… - Nature, 2015 - nature.com
The reactive species of oxygen and chlorine damage cellular components, potentially
leading to cell death. In proteins, the sulfur-containing amino acid methionine is converted to …
leading to cell death. In proteins, the sulfur-containing amino acid methionine is converted to …
The calmodulin‐sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia col
The adenylate cyclase toxin of the prokaryote Bordetella pertussis is stimulated by the
eukaryotic regulatory protein, calmodulin. A general strategy, using the adenylate‐cyclase …
eukaryotic regulatory protein, calmodulin. A general strategy, using the adenylate‐cyclase …
[HTML][HTML] Transgenic tobacco expressing a foreign calmodulin gene shows an enhanced production of active oxygen species
SA Harding, SH Oh, DM Roberts - The EMBO Journal, 1997 - embopress.org
A strategy for elucidating specific molecular targets of calcium and calmodulin in plant
defense responses has been developed. We have used a dominant‐acting calmodulin …
defense responses has been developed. We have used a dominant‐acting calmodulin …
A novel calmodulin‐binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings
E Perruc, M Charpenteau, BC Ramirez… - The Plant …, 2004 - Wiley Online Library
A clone for a novel A rabidopsis t haliana calmodulin (CaM)‐b inding p rotein of 25 kDa
(AtCaMBP25) has been isolated by using a radiolabelled CaM probe to screen a cDNA …
(AtCaMBP25) has been isolated by using a radiolabelled CaM probe to screen a cDNA …
Calmodulin binding domains: characterization of a phosphorylation and calmodulin binding site from myosin light chain kinase
TJ Lukas, WH Burgess, FG Prendergast, W Lau… - Biochemistry, 1986 - ACS Publications
A protein kinase phosphorylation site in chicken gizzard myosin light chain kinase (MLCK)
has been identified, and a synthetic peptide analogue of this site has been shown to be a …
has been identified, and a synthetic peptide analogue of this site has been shown to be a …