Theoretical Aspects and Applications of Aqueous Two‐Phase Systems

M Singla, N Sit - ChemBioEng Reviews, 2023 - Wiley Online Library
The aqueous two‐phase system (ATPS) is commonly known as a technique that yields high‐
purity products in a single step. It is particularly advantageous for purifying biomolecules like …

Development of deep eutectic solvent-based aqueous biphasic system for the extraction of lysozyme

P Xu, Y Wang, J Chen, X Wei, W Xu, R Ni, J Meng… - Talanta, 2019 - Elsevier
Abstract Aqueous Biphasic Systems (ABSs) based on deep eutectic solvents (DESs) were
determined and applied in the extraction of lysozyme from chicken egg white …

[图书][B] Enhancing extraction processes in the food industry

N Lebovka, E Vorobiev, F Chemat - 2012 - api.taylorfrancis.com
Extraction has been used probably since the discovery of fire. Egyptians and Phoenicians,
Jews and Arabs, Indians and Chinese, Greeks and Romans, and even Mayans and Aztecs …

Extractive fermentation for improved production and recovery of lipase derived from Burkholderia cepacia using a thermoseparating polymer in aqueous two-phase …

PL Show, CP Tan, MS Anuar, A Ariff, YA Yusof… - Bioresource …, 2012 - Elsevier
An extractive fermentation technique was developed using a thermoseparating reagent to
form a two-phase system for simultaneous cell cultivation and downstream processing of …

Extractive fermentation using aqueous two-phase systems for integrated production and purification of extracellular lipase derived from Burkholderia pseudomallei

CW Ooi, SL Hii, SMM Kamal, A Ariff, TC Ling - Process Biochemistry, 2011 - Elsevier
An extractive fermentation using aqueous two-phase partitioning system was developed in
this study for the simultaneous cell cultivation and downstream processing of extracellular …

Green, aqueous two-phase systems based on cholinium aminoate ionic liquids with tunable hydrophobicity and charge density

CP Song, RN Ramanan… - ACS Sustainable …, 2015 - ACS Publications
Cholinium-based ionic liquids made of aminoate anions ([Ch][AA] ILs) have been proven to
have low toxicity and to be readily biodegradable. This class of ILs has great potential as a …

Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation

PL Show, CP Tan, MS Anuar, A Ariff, YA Yusof… - Separation and …, 2011 - Elsevier
In this paper, aqueous two-phase flotation (ATPF) composed of thermo-sensitive ethylene
oxide–propylene oxide (EOPO) copolymer and ammonium sulfate was developed for direct …

Aqueous two-phase flotation for the recovery of biomolecules

SY Lee, I Khoiroh, TC Ling, PL Show - Separation & Purification …, 2016 - Taylor & Francis
Aqueous two-phase flotation (ATPF), a novel technique which integrates the principles of
aqueous two-phase systems and mass transfer mode of solvent sublation, has been …

Integration process for protein extraction from microalgae using liquid biphasic electric flotation (LBEF) system

R Sankaran, PL Show, YS Cheng, Y Tao, X Ao… - Molecular …, 2018 - Springer
Microalgae are the most promising sources of protein, which have high potential due to their
high-value protein content. Conventional methods of protein harnessing required multiple …

Enhanced recovery of lipase derived from Burkholderia cepacia from fermentation broth using recyclable ionic liquid/polymer-based aqueous two-phase systems

SY Lee, I Khoiroh, TC Ling, PL Show - Separation and Purification …, 2017 - Elsevier
In this work, a microbial lipase recovery scheme based on aqueous two-phase systems
(ATPS) formed by a biocompatible ionic liquid (IL) and a thermo-sensitive polymer was …