Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: A review based on the separation characteristics of the …

T Ikegami - Journal of separation science, 2019 - Wiley Online Library
Applications of hydrophilic interaction chromatography for the analysis of biopharmaceutical
drugs, ie, glycosylated proteins represented by monoclonal antibodies are discussed in the …

Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues

SE Williams, M Noel, S Lehoux, M Cetinbas… - Nature …, 2022 - nature.com
Glycosylation is essential to brain development and function, but prior studies have often
been limited to a single analytical technique and excluded region-and sex-specific analyses …

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

DJ Harvey - Mass Spectrometry Reviews, 2023 - Wiley Online Library
This review is the tenth update of the original article published in 1999 on the application of
matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry …

Using micro pillar array columns (μPAC) for the analysis of permethylated glycans

BG Cho, P Jiang, M Goli, S Gautam, Y Mechref - Analyst, 2021 - pubs.rsc.org
Glycosylation is a complex and common post-translational modification of proteins. To study
glycosylation, liquid chromatography-mass spectrometry (LC-MS) is often used to profile and …

Glucose unit index (GUI) of permethylated glycans for effective identification of glycans and glycan isomers

S Gautam, W Peng, BG Cho, Y Huang, A Banazadeh… - Analyst, 2020 - pubs.rsc.org
Retention time is the most common and widely used criterion to report the separation of
glycans using Liquid Chromatography (LC), but it varies widely across different columns …

Separation of Permethylated O-Glycans, Free Oligosaccharides, and Glycosphingolipid-Glycans Using Porous Graphitized Carbon (PGC) Column

BG Cho, W Peng, Y Mechref - Metabolites, 2020 - mdpi.com
Glycosylation is one of the most common and complex post-translational modifications of
proteins. However, there are other carbohydrates such as free oligosaccharides and …

Mass spectrometry-based qualitative and quantitative N-glycomics: An update of 2017–2018

K Xiao, Y Han, H Yang, H Lu, Z Tian - Analytica chimica acta, 2019 - Elsevier
N-glycosylation is one of the most frequently occurring protein post-translational
modifications (PTMs) with broad cellular, physiological and pathological relevance. Mass …

Mass Spectrometry Strategies for O-Glycoproteomics

A Helms, JS Brodbelt - Cells, 2024 - mdpi.com
Glycoproteomics has accelerated in recent decades owing to numerous innovations in the
analytical workflow. In particular, new mass spectrometry strategies have contributed to …

Mass Spectrometry Strategies for O-Glycoproteomics. Cells 2024, 13, 394

A Helms, JS Brodbelt - 2024 - europepmc.org
Glycoproteomics has accelerated in recent decades owing to numerous innovations in the
analytical workflow. In particular, new mass spectrometry strategies have contributed to …

The restricted nature of protein glycosylation in the mammalian brain

SE Williams, M Noel, S Lehoux, M Cetinbas, RJ Xavier… - bioRxiv, 2020 - biorxiv.org
Glycosylation is essential to brain development and function, though prior studies have often
been limited to a single analytical technique. Using several methodologies, we analyzed …