Much of our current understanding of microbiology is based on the application of genetic
engineering procedures. Since their inception (more than 30 years ago), methods based …

Allelic exchange is an efficient method of bacterial genome engineering. This protocol
describes the use of this technique to make gene knockouts and knock-ins, as well as single …

An improved method for gene replacement in Pseudomonas aeruginosa was developed.
The method employs several new gene replacement vectors that incorporate (1) the …

The majority of cystic fibrosis (CF) patients succumb to a chronic infection of the airway with
Pseudomonas aeruginosa. Paradoxically, pathogenic strains are often susceptible to …

The Flp-FRT site-specific recombination system from Saccharomyces cerevisiae is a
powerful and efficient tool for high-throughput genetic analysis of bacteria in the …

Two seven-gene phenazine biosynthetic loci were cloned from Pseudomonas aeruginosa
PAO1. The operons, designated phzA1B1C1D1E1F1G1 and phzA2B2C2D2E2F2G2, are …

A set of vector plasmids which greatly facilitate gene replacement and reverse genetics in
many Gram-negative bacteria was constructed. These vectors are based on the P15A origin …

During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa
undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of …

The genome of the soil bacterium Pseudomonas putida strain KT2440 has been erased of
various determinants of resistance to antibiotics encoded in its extant chromosome. To this …

Bacteria growing in biofilms often develop multicellular, three-dimensional structures known
as microcolonies. Complex differentiation within biofilms of Pseudomonas aeruginosa …