In vitro cell cultures are crucial research tools for modeling human development and
diseases. Although the conventional monolayer cell cultures have been widely used in the …

Skeletal muscle is the largest tissue in the body and loss of its function or its regenerative
properties results in debilitating musculoskeletal disorders. Understanding the mechanisms …

Neurons in the cerebral cortex connect through descending pathways to hindbrain and
spinal cord to activate muscle and generate movement. Although components of this …

Integrated in vitro models of human organogenesis are needed to elucidate the multi-
systemic events underlying development and disease. Here we report the generation of …

Neuromuscular networks assemble during early human embryonic development and are
essential for the control of body movement. Previous neuromuscular junction modeling …

INTRODUCTION What determines the pace of embryonic development? Although the
molecular and cellular mechanisms of many developmental processes are evolutionarily …

R-loops are three-stranded nucleic acid structures that accumulate on chromatin in
neurological diseases and cancers and contribute to genome instability. Using a proximity …

The vertebrate spinal cord comprises multiple functionally distinct neuronal cell types
arranged in characteristic positions. During development, these different types of neurons …

Fate decisions in developing tissues involve cells transitioning between discrete cell states,
each defined by distinct gene expression profiles. The Waddington landscape, in which the …

Human pluripotent stem cells (hPSCs) provide a model to study early neural development,
model pathological processes, and develop therapeutics. The generation of functionally …