FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
Rate theories for biologists
HX Zhou - Quarterly reviews of biophysics, 2010 - cambridge.org
Some of the rate theories that are most useful for modeling biological processes are
reviewed. By delving into some of the details and subtleties in the development of the …
reviewed. By delving into some of the details and subtleties in the development of the …
MutS switches between two fundamentally distinct clamps during mismatch repair
Single-molecule trajectory analysis has suggested DNA repair proteins may carry out a one-
dimensional (1D) search on naked DNA encompassing> 10,000 nucleotides. Organized …
dimensional (1D) search on naked DNA encompassing> 10,000 nucleotides. Organized …
Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis
Histograms of single-molecule Förster resonance energy transfer (FRET) efficiency are often
used to study the structures of biomolecules and relate these structures to function. Methods …
used to study the structures of biomolecules and relate these structures to function. Methods …
Structural basis of human PCNA sliding on DNA
M De March, N Merino, S Barrera-Vilarmau… - Nature …, 2017 - nature.com
Sliding clamps encircle DNA and tether polymerases and other factors to the genomic
template. However, the molecular mechanism of clamp sliding on DNA is unknown. Using …
template. However, the molecular mechanism of clamp sliding on DNA is unknown. Using …
Proliferating cell nuclear antigen uses two distinct modes to move along DNA
Proliferating cell nuclear antigen (PCNA) plays an important role in eukaryotic genomic
maintenance by topologically binding DNA and recruiting replication and repair proteins …
maintenance by topologically binding DNA and recruiting replication and repair proteins …
The RFC clamp loader: structure and function
NY Yao, M O'Donnell - The Eukaryotic Replisome: A Guide to Protein …, 2012 - Springer
The eukaryotic RFC clamp loader couples the energy of ATP hydrolysis to open and close
the circular PCNA sliding clamp onto primed sites for use by DNA polymerases and repair …
the circular PCNA sliding clamp onto primed sites for use by DNA polymerases and repair …
Structure of p15PAF–PCNA complex and implications for clamp sliding during DNA replication and repair
A De Biasio, AI De Opakua, GB Mortuza… - Nature …, 2015 - nature.com
The intrinsically disordered protein p15PAF regulates DNA replication and repair by binding
to the proliferating cell nuclear antigen (PCNA) sliding clamp. We present the structure of the …
to the proliferating cell nuclear antigen (PCNA) sliding clamp. We present the structure of the …
Clamp loader ATPases and the evolution of DNA replication machinery
BA Kelch, DL Makino, M O'Donnell, J Kuriyan - BMC biology, 2012 - Springer
Clamp loaders are pentameric ATPases of the AAA+ family that operate to ensure
processive DNA replication. They do so by loading onto DNA the ring-shaped sliding clamps …
processive DNA replication. They do so by loading onto DNA the ring-shaped sliding clamps …
What are the molecular requirements for protein sliding along DNA?
DNA-binding proteins rely on linear diffusion along the longitudinal DNA axis, supported by
their nonspecific electrostatic affinity for DNA, to search for their target recognition sites. One …
their nonspecific electrostatic affinity for DNA, to search for their target recognition sites. One …