Since the first generation of a negative-sense RNA virus entirely from cloned cDNA in 1994,
similar reverse genetics systems have been established for members of most genera of the …

Publisher Summary Influenza viruses are enveloped viruses, often spherical (80 to 120 nm
in diameter) but also filamentous (several micrometers in length). The lipid envelope is …

We have developed an eight-plasmid DNA transfection system for the rescue of infectious
influenza A virus from cloned cDNA. In this plasmid-based expression system, viral cDNA is …

We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight
individual negative-sense genomic viral RNAs were transcribed from plasmids containing …

For influenza virus, we developed an efficient, noncytotoxic, plasmid-based virus-like
particle (VLP) system to reflect authentic virus particles. This system was characterized …

To get insights into the role played by each of the influenza A virus polypeptides in
morphogenesis and virus particle assembly, the generation of virus-like particles (VLPs) has …

We are studying the structural proteins and molecular interactions required for formation and
release of influenza virus-like particles (VLPs) from the cell surface. To investigate these …

Avian influenza viruses represent a growing threat for an influenza pandemic. To develop
recombinant vaccine for avian influenza of the H9N2 subtype, we expressed in insect cells …

The cytoplasmic tail of the influenza A virus M2 protein is highly conserved among influenza
A virus isolates. The cytoplasmic tail appears to be dispensable with respect to the ion …

The M2 integral membrane protein encoded by influenza A virus possesses an ion channel
activity that is required for efficient virus entry into host cells. The role of the M2 protein …