The recently discovered TPR gene family encodes a diverse group of proteins that function
in mitosis, transcription, splicing, protein import and neurogenesis. These multi-domain …

Mitosis is the process by which eukaryotic cells organize and segregate their chromosomes
in preparation for cell division. It is accomplished by a cellular machine composed largely of …

Spindle microtubules (MTs) in PtK1 cells, fixed at stages from metaphase to telophase, have
been reconstructed using serial sections, electron microscopy, and computer image …

Spindle formation in fission yeast occurs by the interdigitation of two microtubule arrays
extending from duplicated spindle pole bodies which span the nuclear membrane. By …

Mitotic spindles of Schizosaccharomyces pombe have been studied by EM, using serial
cross sections to reconstruct 12 spindles from cells that were ultrarapidly frozen and fixed by …

The S. pombe nuc2+ gene is required for mitotic chromosome disjunction. Its mutation
arrests mitosis at the metaphase. The gene product is present in the nuclear scaffold-like …

SEVERAL mitotic and meiotic gene products are related to the microtubule motor kinesin,
providing insight into the molecular basis of the complex motile events responsible for …

ABSTRACT γ-Tubulin exists in fission yeast as the product of an essential gene, encoding a
446 amino acid protein that is 77.3% identical to Aspergillus nidulans γ-tubulin. The gene …

Members of the MKLP1 subfamily of kinesin motor proteins localize to the equatorial region
of the spindle midzone and are capable of bundling antiparallel microtubules in vitro …

Yeast spindle pole bodies (SPBs) with attached nuclear microtubles were enriched
approximately 600-fold from yeast cell extracts. 14 mAbs prepared against this enriched …