Novel strategies to address the challenges in top-down proteomics
JA Melby, DS Roberts, EJ Larson… - Journal of the …, 2021 - ACS Publications
Top-down mass spectrometry (MS)-based proteomics is a powerful technology for
comprehensively characterizing proteoforms to decipher post-translational modifications …
comprehensively characterizing proteoforms to decipher post-translational modifications …
Identification and quantification of proteoforms by mass spectrometry
A proteoform is a defined form of a protein derived from a given gene with a specific amino
acid sequence and localized post‐translational modifications. In top‐down proteomic …
acid sequence and localized post‐translational modifications. In top‐down proteomic …
[HTML][HTML] Identification of splice variants and isoforms in transcriptomics and proteomics
T Su, MAR Hollas, RT Fellers… - Annual review of …, 2023 - annualreviews.org
Alternative splicing is pivotal to the regulation of gene expression and protein diversity in
eukaryotic cells. The detection of alternative splicing events requires specific omics …
eukaryotic cells. The detection of alternative splicing events requires specific omics …
High-throughput quantitative top-down proteomics
KA Cupp-Sutton, S Wu - Molecular omics, 2020 - pubs.rsc.org
Top-down mass spectrometry (MS) analyzes intact proteins at the proteoform level, which
allows researchers to better understand the functions of protein modifications. Recently, top …
allows researchers to better understand the functions of protein modifications. Recently, top …
Ion activation methods for peptides and proteins
Mass spectrometry is well-established as one of the primary methods used to characterize
peptides and proteins. New advances in instrumentation and innovative analytical workflows …
peptides and proteins. New advances in instrumentation and innovative analytical workflows …
Native top‐down mass spectrometry for higher‐order structural characterization of proteins and complexes
R Liu, S Xia, H Li - Mass spectrometry reviews, 2023 - Wiley Online Library
Progress in structural biology research has led to a high demand for powerful and yet
complementary analytical tools for structural characterization of proteins and protein …
complementary analytical tools for structural characterization of proteins and protein …
[HTML][HTML] Structure and dynamics of endogenous cardiac troponin complex in human heart tissue captured by native nanoproteomics
Protein complexes are highly dynamic entities that display substantial diversity in their
assembly, post-translational modifications, and non-covalent interactions, allowing them to …
assembly, post-translational modifications, and non-covalent interactions, allowing them to …
Seeing the complete picture: proteins in top-down mass spectrometry
Top-down protein mass spectrometry can provide unique insights into protein sequence and
structure, including precise proteoform identification and study of protein–ligand and protein …
structure, including precise proteoform identification and study of protein–ligand and protein …
Structural O-glycoform heterogeneity of the SARS-CoV-2 spike protein receptor-binding domain revealed by top-down mass spectrometry
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively
glycosylated surface spike (S) protein to mediate host cell entry, and the S protein …
glycosylated surface spike (S) protein to mediate host cell entry, and the S protein …
Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics of Mouse Brain Integral Membrane Proteins
Mass spectrometry (MS)-based top-down characterization of integral membrane proteins
(IMPs) is crucial for understanding their functions in biological processes. However, it is …
(IMPs) is crucial for understanding their functions in biological processes. However, it is …