Photodissociation mass spectrometry combines the ability to activate and fragment ions
using photons with the sensitive detection of the resulting product ions by mass …

Introduction The N-terminus of a protein can encode several protein features, including its
half-live and its localization. As the proteomics field remains dominated by bottom-up …

A kiloelectronvolt beam of helium ions is used to ionize and fragment precursor peptide ions
starting in the 1+ charge state. The electron affinity of helium cations (24.6 eV) exceeds the …

Six ion fragmentation techniques that can distinguish aspartic acid from its isomer,
isoaspartic acid, were compared. MALDI post-source decay (PSD), MALDI 157 nm …

In silico gene prediction has proven to be prone to errors, especially regarding precise
localization of start codons that spread in subsequent biological studies. Therefore, the high …

Protein therapeutics are susceptible to clipping via enzymatic and nonenzymatic
mechanisms that create neo-N-termini. Typically, neo-N-termini are identified by chemical …

The fragmentation behavior of three peptides (RGAFSTFGAS, GAFSTFGASR, and
GAFSTFGASS) was evaluated by collisional activated dissociation (CAD), higher energy …

The first application of light‐emitting diodes (LEDs) for ultraviolet photodissociation (UVPD)
mass spectrometry is reported. LEDs provide a compact, low cost light source and have …

Charge tagging is a peptide derivatization process that commonly localizes a positive
charge on the N-terminus. Upon low energy activation (eg, collision-induced dissociation or …

Rationale Proteins undergo post‐translational modifications and proteolytic processing that
can affect their biological function. Processing often involves the loss of single residues …