[HTML][HTML] Reprint of “Mixed-mode chromatography in pharmaceutical and biopharmaceutical applications”

K Zhang, X Liu - Journal of pharmaceutical and biomedical analysis, 2016 - Elsevier
Mixed-mode chromatography (MMC) is a fast growing area in recent years, thanks to the
new generation of mixed-mode stationary phases and better understanding of multimode …

The use of predictive models to develop chromatography-based purification processes

CR Bernau, M Knödler, J Emonts, RC Jäpel… - … in Bioengineering and …, 2022 - frontiersin.org
Chromatography is the workhorse of biopharmaceutical downstream processing because it
can selectively enrich a target product while removing impurities from complex feed streams …

Evaluating antibody monomer separation from associated aggregates using mixed-mode chromatography

D Gao, LL Wang, DQ Lin, SJ Yao - Journal of Chromatography A, 2013 - Elsevier
Aggregate level is a key factor when assessing quality attributes of antibody product, since
the aggregation might impact the biological activity of biopharmaceutical. Effective …

Development of multimodal membrane adsorbers for antibody purification using atom transfer radical polymerization

J Wang, RT Sproul, LS Anderson, SM Husson - Polymer, 2014 - Elsevier
This contribution describes a graft polymerization strategy to prepare multimodal
membranes, a new class of high-productivity adsorptive materials for the purification of …

Strong cation-exchange chromatography of proteins on a sulfoalkylated monolithic cryogel

I Perçin, R Khalaf, B Brand, M Morbidelli… - … of Chromatography A, 2015 - Elsevier
A new strong cation exchanger (SCX) monolithic column was synthesized by at-line surface
modification of a cryogel prepared by copolymerization of 2-hydroxyethylmethacrylate …

Chromatographic parameter determination for complex biological feedstocks

SM Pirrung, D Parruca da Cruz, AT Hanke… - Biotechnology …, 2018 - Wiley Online Library
The application of mechanistic models for chromatography requires accurate model
parameters. Especially for complex feedstocks such as a clarified cell harvest, this can still …

Computational engineering of protein L to achieve an optimal affinity chromatography resin for purification of antibody fragments

S Rahmati, F Torkashvand, M Amanlou… - Analytical …, 2021 - ACS Publications
Protein L affinity chromatography is a useful method for the purification of antibody
fragments containing kappa light chains. In affinity chromatography, increasing the binding …

Carboxymethylcellulose/pectin inhibiting structural folding of rice proteins via trinary structural interplays

Y Yang, R Wang, W Feng, X Zhou, Z Chen… - International journal of …, 2019 - Elsevier
Developing food components combining materials properties from both proteins and
polysaccharides is an intriguing as well as challenging task in food industry. In the present …

Calixarene-engineered surfaces and separation science

O Gezici, M Bayrakci - Journal of Inclusion Phenomena and Macrocyclic …, 2015 - Springer
After their discovery, calixarenes have attracted the attention of many researchers from
various disciplines. This group of supramolecules has an increasing popularity and this is …

L-histidine functionalized multi-walled carbon nanotubes for on-line affinity separation and purification of immunoglobulin G in serum

Z Du, S Zhang, C Zhou, M Liu, G Li - Talanta, 2012 - Elsevier
In this work, the multi-walled carbon nanotubes were covalently functionalized with l-
histidine (His–MWNTs) as online pseudospecific affinity adsorbent for immunoglobulin G …