α-glucosidase is a major enzyme that is involved in starch digestion and type 2 diabetes
mellitus. In this study, the inhibition of hypericin by α-glucosidase and its mechanism were …

Monolayer graphene field-effect sensors operating in liquid have been widely deployed for
detecting a range of analyte species often under equilibrium conditions. Here we report on …

Flavonoids, a class of natural drugs with broad biological activity, exhibit inhibitory effect on
α-amylase. Citrus peel is a good source of flavonoids. The real-time interactions between …

Butyrylcholinesterase (BChE) plays a pivotal role in regulating acetylcholine (ACh) levels
during the progression of Alzheimer's disease (AD), so emerged as an attractive target in AD …

Background Surface plasmon resonance is a label-free biophysical technique that is widely
used in investigating biomolecular interactions, including protein-protein, protein-DNA, and …

DNA topoisomerases are essential enzymes for all living organisms and important targets
for anticancer drugs and antibiotics. Although DNA topoisomerases have been studied …

Escherichia coli topoisomerase I (EctopoI), a type IA DNA topoisomerase, relaxes the
negative DNA supercoiling generated by RNA polymerase (RNAP) during transcription …

Analyzing surface plasmon resonance data: Choosing a correct biphasic model for
interpretation | Review of Scientific Instruments | AIP Publishing Skip to Main Content …

This study evaluated the interaction of Chrysanthemum indicum (CI) flavonoids (luteolin,
acacetin, and buddleoside) with α‐amylase. Surface plasmon resonance (SPR) assay …

Surface plasmon resonance (SPR) is a widely used, affinity based, label-free biophysical
technique to investigate biomolecular interactions. The extraction of rate constants requires …