Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …

Principle has it that even the most advanced super‐resolution microscope would be futile in
providing biological insight into subcellular matrices without well‐designed fluorescent …

Super-resolution techniques have begun to transform biological and biomedical research by
allowing researchers to observe structures well below the classic diffraction limit of light …

Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular
scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent …

Tumors constitute highly suppressive microenvironments in which infiltrating T cells are"
exhausted" by inhibitory receptors such as PD-1. Here we identify TIGIT as a coinhibitory …

All life forms have an absolute requirement for metals, as metals play critical roles in
fundamental processes, including osmotic regulation, catalysis, metabolism …

Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

Uncovering the mechanisms of virus infection and assembly is crucial for preventing the
spread of viruses and treating viral disease. The technique of single-virus tracking (SVT) …

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can
be implemented flexibly in living cells and in vivo. However, the design of synthetic …

Actin functions in a multitude of cellular processes owing to its ability to polymerize into
filaments, which can be further organized into higher-order structures by an array of actin …