Paramagnetic chemical probes for studying biological macromolecules
Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …
Synthesis of nucleobase-modified RNA oligonucleotides by post-synthetic approach
K Bartosik, K Debiec, A Czarnecka, E Sochacka… - Molecules, 2020 - mdpi.com
The chemical synthesis of modified oligoribonucleotides represents a powerful approach to
study the structure, stability, and biological activity of RNAs. Selected RNA modifications …
study the structure, stability, and biological activity of RNAs. Selected RNA modifications …
Long-range distance determination in fully deuterated RNA with pulsed EPR spectroscopy
Pulsed electron-electron double resonance (PELDOR or DEER) spectroscopy is powerful in
structure and dynamics study of biological macromolecules by providing distance …
structure and dynamics study of biological macromolecules by providing distance …
Site selective and efficient spin labeling of proteins with a maleimide-functionalized trityl radical for pulsed dipolar EPR spectroscopy
Pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) in combination with
site-directed spin labeling (SDSL) of proteins and oligonucleotides is a powerful tool in …
site-directed spin labeling (SDSL) of proteins and oligonucleotides is a powerful tool in …
Recent advances in RNA structurome
B Xu, Y Zhu, C Cao, H Chen, Q Jin, G Li, J Ma… - Science China Life …, 2022 - Springer
RNA structures are essential to support RNA functions and regulation in various biological
processes. Recently, a range of novel technologies have been developed to decode …
processes. Recently, a range of novel technologies have been developed to decode …
Do the P1 and P2 hairpins of the Guanidine-II riboswitch interact?
Riboswitches regulate genes by adopting different structures in responds to metabolite
binding. The guanidine-II riboswitch is the smallest representative of the ykkC class with the …
binding. The guanidine-II riboswitch is the smallest representative of the ykkC class with the …
Biomolecular EPR meets NMR at high magnetic fields
In this review on advanced biomolecular EPR spectroscopy, which addresses both the EPR
and NMR communities, considerable emphasis is put on delineating the complementarity of …
and NMR communities, considerable emphasis is put on delineating the complementarity of …
Site-Directed Spin Labeling of RNA with a Gem-Diethylisoindoline Spin Label: PELDOR, Relaxation, and Reduction Stability
C Wuebben, S Blume, D Abdullin, D Brajtenbach… - Molecules, 2019 - mdpi.com
Ribonucleic acid function is governed by its structure, dynamics, and interaction with other
biomolecules and influenced by the local environment. Thus, methods are needed that …
biomolecules and influenced by the local environment. Thus, methods are needed that …
A review on recent advances in methods for site-directed spin labeling of long RNAs
K Huang, X Fang - International Journal of Biological Macromolecules, 2023 - Elsevier
RNAs are important biomolecules that play essential roles in various cellular processes and
are crucially linked with many human diseases. The key to elucidate the mechanisms …
are crucially linked with many human diseases. The key to elucidate the mechanisms …
Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions
Site-directed spin labeling (SDSL) of large RNAs for electron paramagnetic resonance
(EPR) spectroscopy has remained challenging to date. We here demonstrate an efficient …
(EPR) spectroscopy has remained challenging to date. We here demonstrate an efficient …