Recent advances and state-of-the-art strategies in strain and process engineering for biobutanol production by Clostridium acetobutylicum

C Xue, J Zhao, L Chen, ST Yang, F Bai - Biotechnology advances, 2017 - Elsevier
Butanol as an advanced biofuel has gained great attention due to its environmental benefits
and superior properties compared to ethanol. However, the cost of biobutanol production via …

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

S Li, L Huang, C Ke, Z Pang, L Liu - Biotechnology for biofuels, 2020 - Springer
The global energy crisis and limited supply of petroleum fuels have rekindled the interest in
utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a …

CRISPR‐based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii

Q Li, J Chen, NP Minton, Y Zhang, Z Wen… - Biotechnology …, 2016 - Wiley Online Library
Solventogenic clostridia are important industrial microorganisms that produce various
chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both …

Recent Developments of the Synthetic Biology Toolkit for Clostridium

RC Joseph, NM Kim, NR Sandoval - Frontiers in microbiology, 2018 - frontiersin.org
The Clostridium genus is a large, diverse group consisting of Gram-positive, spore-forming,
obligate anaerobic firmicutes. Among this group are historically notorious pathogens as well …

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium

Z Wen, NP Minton, Y Zhang, Q Li, J Liu, Y Jiang… - Metabolic …, 2017 - Elsevier
The efficient fermentative production of solvents (acetone, n-butanol, and ethanol) from a
lignocellulosic feedstock using a single process microorganism has yet to be demonstrated …

[HTML][HTML] A roadmap for gene system development in Clostridium

NP Minton, M Ehsaan, CM Humphreys, GT Little… - Anaerobe, 2016 - Elsevier
Clostridium species are both heroes and villains. Some cause serious human and animal
diseases, those present in the gut microbiota generally contribute to health and wellbeing …

[HTML][HTML] A two-plasmid inducible CRISPR/Cas9 genome editing tool for Clostridium acetobutylicum

F Wasels, J Jean-Marie, F Collas… - Journal of …, 2017 - Elsevier
CRISPR/Cas-based genetic engineering has revolutionised molecular biology in both
eukaryotes and prokaryotes. Several tools dedicated to the genomic transformation of the …

Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes in Corynebacterium glutamicum

Y Liu, R Wang, J Liu, H Lu, H Li, Y Wang, X Ni, J Li… - Science …, 2022 - science.org
Genome-scale functional screening accelerates comprehensive assessment of gene
function in cells. Here, we have established a genome-scale loss-of-function screening …

Sporulation in solventogenic and acetogenic clostridia

M Diallo, SWM Kengen… - Applied Microbiology and …, 2021 - Springer
The Clostridium genus harbors compelling organisms for biotechnological production
processes; while acetogenic clostridia can fix C1-compounds to produce acetate and …

CRISPR–Cas9D10A nickase‐assisted base editing in the solvent producer Clostridium beijerinckii

Q Li, FM Seys, NP Minton, J Yang… - Biotechnology and …, 2019 - Wiley Online Library
Clostridium beijerinckii is a potentially important industrial microorganism as it can
synthesize valuable chemicals and fuels from various carbon sources. The establishment of …