Next-generation physiology approaches to study microbiome function at single cell level

R Hatzenpichler, V Krukenberg, RL Spietz… - Nature Reviews …, 2020 - nature.com
The function of cells in their native habitat often cannot be reliably predicted from genomic
data or from physiology studies of isolates. Traditional experimental approaches to study the …

The microbial rare biosphere: current concepts, methods and ecological principles

F Pascoal, R Costa, C Magalhães - FEMS Microbiology Ecology, 2021 - academic.oup.com
Our ability to describe the highly diverse pool of low abundance populations present in
natural microbial communities is increasing at an unprecedented pace. Yet we currently lack …

Active antibiotic resistome in soils unraveled by single-cell isotope probing and targeted metagenomics

HZ Li, K Yang, H Liao, SB Lassen… - Proceedings of the …, 2022 - National Acad Sciences
Antimicrobial resistance (AMR) in soils represents a serious risk to human health through
the food chain and human–nature contact. However, the active antibiotic-resistant bacteria …

Diversity of cellulolytic microorganisms and microbial cellulases

L Liu, WC Huang, Y Liu, M Li - International Biodeterioration & …, 2021 - Elsevier
Lignocellulosic biomass is a geographically abundant source of renewable energy, showing
enormous potential to contribute to escalating energy demands. Improving the industrial …

Importance of substrate quality and clay content on microbial extracellular polymeric substances production and aggregate stability in soils

FK Olagoke, A Bettermann, PTB Nguyen… - Biology and Fertility of …, 2022 - Springer
We investigated the effects of substrate (cellulose or starch) and different clay contents on
the production of microbial extracellular polymeric substances (EPS) and concomitant …

Single-cell exploration of active phosphate-solubilizing bacteria across diverse soil matrices for sustainable phosphorus management

HZ Li, J Peng, K Yang, Y Zhang, QL Chen, YG Zhu… - Nature Food, 2024 - nature.com
Phosphate-solubilizing bacteria (PSB) are crucial for enhancing phosphorus bioavailability
and regulating phosphorus transformation processes. However, the in situ phosphorus …

Halving gypsum dose by Penicillium oxalicum on alkaline neutralization and microbial community reconstruction in bauxite residue

Y Jiang, X Qin, F Zhu, Y Zhang, XC Zhang… - Chemical Engineering …, 2023 - Elsevier
Bauxite residue is a high-alkaline solid waste generated during alumina production, which
greatly limits the growth of soil microorganisms and plants. Here, we developed a novel …

Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting

L Dridi, F Altamura, E Gonzalez, O Lui… - Nature …, 2023 - nature.com
The composition and metabolism of the human gut microbiota are strongly influenced by
dietary complex glycans, which cause downstream effects on the physiology and health of …

Microbial single-cell omics: the crux of the matter

AK Kaster, MS Sobol - Applied Microbiology and Biotechnology, 2020 - Springer
Single-cell genomics and transcriptomics can provide reliable context for assembled
genome fragments and gene expression activity on the level of individual prokaryotic …

Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota

NO Nou, JK Covington, D Lai, X Mayali… - Nature …, 2024 - nature.com
Few aerobic hyperthermophilic microorganisms degrade polysaccharides. Here, we
describe the genome-enabled enrichment and optical tweezer-based isolation of an aerobic …