Protein-protein interactions are fundamental to the formation of intricate interaction networks
and the assembly of multisubunit protein complexes that represent the functional …

Crosslinking mass spectrometry (crosslinking-MS) is a versatile tool providing structural
insights into protein conformation and protein-protein interactions. Its medium-resolution …

Cellular functions are governed by molecular machines that assemble through protein-
protein interactions. Their atomic details are critical to studying their molecular mechanisms …

Mitochondrial protein interactions and complexes facilitate mitochondrial function. These
complexes range from simple dimers to the respirasome supercomplex consisting of …

Highlights•Cross-linking-mass spectrometry constitutes a chemical method to produce
protein complex structural model constraints.•A wide range of sample types are applicable …

Crosslinking mass spectrometry (MS) has emerged as an important technique for
elucidating the in-solution structures of protein complexes and the topology of protein …

While modern structural biology technologies have greatly expanded the size and type of
protein complexes that can now be studied, the ability to derive large-scale structural …

Knowledge of protein structure is paramount to the understanding of biological function,
developing new therapeutics, and making detailed mechanistic hypotheses. Therefore …

Combining diverse experimental structural and interactomic methods allows for the
construction of comprehensible molecular encyclopedias of biological systems. Typically …

This protocol describes a workflow for utilizing large-scale cross-linking with mass
spectrometry (XL-MS) to make systems-level structural biology measurements in complex …