Fluorescent probes for lipid membranes: from the cell surface to organelles

AS Klymchenko - Accounts of Chemical Research, 2022 - ACS Publications
Conspectus Biomembranes are ubiquitous lipid structures that delimit the cell surface and
organelles and operate as platforms for a multitude of biomolecular processes. The …

Photobleaching of organic fluorophores: quantitative characterization, mechanisms, protection

AP Demchenko - Methods and applications in fluorescence, 2020 - iopscience.iop.org
Photochemical stability is one of the most important parameters that determine the
usefulness of organic dyes in different applications. This Review addresses key factors that …

Targeted solvatochromic fluorescent probes for imaging lipid order in organelles under oxidative and mechanical stress

DI Danylchuk, PH Jouard… - Journal of the American …, 2021 - ACS Publications
Biomembranes constitute a basis for all compartments of live cells, and therefore, the
monitoring of their lipid organization is essential for understanding cell status and activity …

Design and application of fluorescent probes to detect cellular physical microenvironments

J Ma, R Sun, K Xia, Q Xia, Y Liu, X Zhang - Chemical Reviews, 2024 - ACS Publications
The microenvironment is indispensable for functionality of various biomacromolecules,
subcellular compartments, living cells, and organisms. In particular, physical properties …

Solvatochromic and fluorogenic dyes as environment-sensitive probes: design and biological applications

AS Klymchenko - Accounts of chemical research, 2017 - ACS Publications
Conspectus Fluorescent environment-sensitive probes are specially designed dyes that
change their fluorescence intensity (fluorogenic dyes) or color (eg, solvatochromic dyes) in …

Large Stokes shift benzothiazolium cyanine dyes with improved intramolecular charge transfer (ICT) for cell imaging applications

CS Abeywickrama - Chemical Communications, 2022 - pubs.rsc.org
Intramolecular Charge Transfer (ICT) is a crucial photophysical phenomenon that can be
used to improve the Stokes' shift in fluorescent dyes. The introduction of molecular …

Principles of aggregation‐induced emission: design of deactivation pathways for advanced AIEgens and applications

S Suzuki, S Sasaki, AS Sairi, R Iwai… - Angewandte …, 2020 - Wiley Online Library
Twenty years ago, the concept of aggregation‐induced emission (AIE) was proposed, and
this unique luminescent property has attracted scientific interest ever since. However, AIE …

Ferroptosis mediates cuprizone-induced loss of oligodendrocytes and demyelination

P Jhelum, E Santos-Nogueira, W Teo… - Journal of …, 2020 - Soc Neuroscience
Multiple sclerosis (MS) is a chronic demyelinating disease of the CNS. Cuprizone (CZ), a
copper chelator, is widely used to study demyelination and remyelination in the CNS, in the …

Recent advances in fluorescent probes for lipid droplets

TK Fam, AS Klymchenko, M Collot - Materials, 2018 - mdpi.com
Lipid droplets (LDs) are organelles that serve as the storage of intracellular neutral lipids.
LDs regulate many physiological processes. They recently attracted attention after extensive …

Mechanosensitive fluorescent probes to image membrane tension in mitochondria, endoplasmic reticulum, and lysosomes

A Goujon, A Colom, K Strakova, V Mercier… - Journal of the …, 2019 - ACS Publications
Measuring forces inside cells is particularly challenging. With the development of
quantitative microscopy, fluorophores which allow the measurement of forces became highly …