This review examines how real-time PCR can be used to determine copy number and
zygosity in transgenic plants. Distinguishing between plants that harbor one and two copies …

Abstract Dabing Zhang (Corresponding author) As the worldwide commercialization of
genetically modified organisms (GMOs) increases and consumers concern the safety of …

Background Even though real-time PCR has been broadly applied in biomedical sciences,
data processing procedures for the analysis of quantitative real-time PCR are still lacking; …

Background As engineered biological systems become more complex, it is increasingly
common to express multiple operons from different plasmids and inducible expression …

Drought and high salinity are two major environmental factors that significantly limit the
productivity of agricultural crops worldwide. WRKY transcription factors play essential roles …

Phosphorus (P) remobilization in plants is required for continuous growth and development.
The Arabidopsis (Arabidopsis thaliana) inorganic phosphate (Pi) transporter Pht1; 5 has …

Fruit ripening is a complex process that involves a series of physiological and biochemical
changes that ultimately influence fruit quality traits, such as color and flavor. Sugar …

Background As a large family of regulatory proteins, WRKY transcription factors play
essential roles in the processes of adaptation to diverse environmental stresses and plant …

In transgenic plants, transgene copy number can greatly influence the expression level and
genetic stability of the target gene, making estimation of transgene copy number an …

Key message Our results indicate that overexpression of the GhWRKY39-1 gene enhances
resistance to pathogen infection and tolerance to high salt and oxidative stress in transgenic …