Single-molecule localization microscopy in eukaryotes

M Sauer, M Heilemann - Chemical reviews, 2017 - ACS Publications
Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Imaging live-cell dynamics and structure at the single-molecule level

Z Liu, LD Lavis, E Betzig - Molecular cell, 2015 - cell.com
Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …

Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells

DP Hoffman, G Shtengel, CS Xu, KR Campbell… - Science, 2020 - science.org
INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

The 2015 super-resolution microscopy roadmap

SW Hell, SJ Sahl, M Bates, X Zhuang… - Journal of Physics D …, 2015 - iopscience.iop.org
Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

Stimulated emission depletion microscopy

H Blom, J Widengren - Chemical reviews, 2017 - ACS Publications
Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Cross-strand binding of TFAM to a single mtDNA molecule forms the mitochondrial nucleoid

C Kukat, KM Davies, CA Wurm… - Proceedings of the …, 2015 - National Acad Sciences
Mammalian mitochondrial DNA (mtDNA) is packaged by mitochondrial transcription factor A
(TFAM) into mitochondrial nucleoids that are of key importance in controlling the …

Correlated light and electron microscopy: ultrastructure lights up!

P De Boer, JP Hoogenboom, BNG Giepmans - Nature methods, 2015 - nature.com
Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek
observed living microorganisms and cells in 1674 using his light microscope. A spectrum of …

High-density three-dimensional localization microscopy across large volumes

WR Legant, L Shao, JB Grimm, TA Brown, DE Milkie… - Nature …, 2016 - nature.com
Abstract Extending three-dimensional (3D) single-molecule localization microscopy away
from the coverslip and into thicker specimens will greatly broaden its biological utility …

The maintenance of mitochondrial DNA integrity—critical analysis and update

M Alexeyev, I Shokolenko… - Cold Spring Harbor …, 2013 - cshperspectives.cshlp.org
DNA molecules in mitochondria, just like those in the nucleus of eukaryotic cells, are
constantly damaged by noxious agents. Eukaryotic cells have developed efficient …

Live-cell STED nanoscopy of mitochondrial cristae

T Stephan, A Roesch, D Riedel, S Jakobs - Scientific reports, 2019 - nature.com
Mitochondria are highly dynamic organelles that exhibit a complex inner architecture. They
exhibit a smooth outer membrane and a highly convoluted inner membrane that forms …