Immobilization and purification of enzymes are usual requirements for their industrial use.
Both purification and immobilization have a common factor: they use a solid activated …

A heterofunctional support for enzyme immobilization may be defined as that which
possesses several distinct functionalities on its surface able to interact with a protein. We will …

The goal in biomaterial surface modification is to retain a material's bulk properties while
modifying only its surface to possess desired recognition and specificity. Here we develop a …

Recombinant therapeutic proteins have gained enormous importance for clinical
applications. The first recombinant products have been produced in E. coli more than 20 …

Affinity chromatography is one of the most diverse and powerful chromatographic methods
for purification of a specific molecule or a group of molecules from complex mixtures. It is …

Mixed-mode chromatography is a chromatographic method that utilizes more than one form
of interactions between the stationary phase and the solutes in a feed stream. Compared …

The demand for recombinant therapeutic antibodies and Fc-fusion proteins is expected to
increase in the years to come. Hence, extensive efforts are concentrated on improving the …

A series of small-molecule microbicides has been developed for vaginal delivery to prevent
heterosexual HIV transmission, but results from human clinical trials have been …

Bromelain is a mixture of proteolytic enzymes found in pineapple (Ananas comosus) plants.
It can be found in several parts of the pineapple plant, including the stem, fruit, leaves and …

Biomimetic affinity chromatography with short peptide ligands, as a promising bioseparation
technique, has great potential to protein separation and purification, which is based on …