Photophysical processes in single molecule organic fluorescent probes

EMS Stennett, MA Ciuba, M Levitus - Chemical Society Reviews, 2014 - pubs.rsc.org
The use of organic fluorescent probes in biochemical and biophysical applications of single
molecule spectroscopy and fluorescence microscopy techniques continues to increase. As …

Methods for physical characterization of phase-separated bodies and membrane-less organelles

DM Mitrea, B Chandra, MC Ferrolino, EB Gibbs… - Journal of molecular …, 2018 - Elsevier
Membrane-less organelles are cellular structures which arise through the phenomenon of
phase separation. This process enables compartmentalization of specific sets of …

[HTML][HTML] Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins

R Raghupathy, AA Anilkumar, A Polley, PP Singh… - Cell, 2015 - cell.com
Understanding how functional lipid domains in live cell membranes are generated has
posed a challenge. Here, we show that transbilayer interactions are necessary for the …

Practical guidelines for dual-color fluorescence cross-correlation spectroscopy

K Bacia, P Schwille - Nature protocols, 2007 - nature.com
Dual-color fluorescence cross-correlation spectroscopy (FCCS) allows for the determination
of molecular mobility and concentrations and for the quantitative analysis of molecular …

The nature of the amyloid-β monomer and the monomer-oligomer equilibrium

S Nag, B Sarkar, A Banerjee, B Sahoo, KAS Varun… - Biophysical …, 2011 - cell.com
The monomer to oligomer transition initiates the aggregation and pathogenic transformation
of Alzheimer's Amyloid-β (Aβ) peptide. However, the monomeric state of this aggregation …

Noncovalent cell surface engineering: incorporation of bioactive synthetic glycopolymers into cellular membranes

D Rabuka, MB Forstner, JT Groves… - Journal of the American …, 2008 - ACS Publications
The controlled addition of structurally defined components to live cell membranes can
facilitate the molecular level analysis of cell surface phenomena. Here we demonstrate that …

Development of a rhodamine–rhodanine-based fluorescent mercury sensor and its use to monitor real-time uptake and distribution of inorganic mercury in live …

K Bera, AK Das, M Nag, S Basak - Analytical chemistry, 2014 - ACS Publications
We introduce a new rhodamine–rhodanine-based “turn-on” fluorescent sensor (RR1) and
describe its application for detection of mercury, including in solution, in live cells, and in a …

Diffusivity of asphaltene molecules by fluorescence correlation spectroscopy

AB Andrews, RE Guerra, OC Mullins… - The Journal of Physical …, 2006 - ACS Publications
Using fluorescence correlation spectroscopy (FCS) we measure the translational diffusion
coefficient of asphaltene molecules in toluene at extremely low concentrations (0.03− 3.0 …

The Amyloid β Peptide (Aβ1-40) Is Thermodynamically Soluble at Physiological Concentrations

P Sengupta, K Garai, B Sahoo, Y Shi… - Biochemistry, 2003 - ACS Publications
Precipitation of the 39− 43-residue amyloid β peptide (Aβ) is a crucial factor in Alzheimer's
disease (AD). In normal as well as in AD-afflicted brain, the Aβ concentration is estimated to …

Significant structural differences between transient amyloid‐β oligomers and less‐toxic fibrils in regions known to harbor familial alzheimer′ s mutations

B Sarkar, VS Mithu, B Chandra, A Mandal… - Angewandte …, 2014 - Wiley Online Library
Small oligomers of the amyloid β (Aβ) peptide, rather than the monomers or the fibrils, are
suspected to initiate Alzheimer′ s disease (AD). However, their low concentration and …