Simple Summary Next-generation sequencing (NGS) is a powerful tool used in genomics
research. NGS can sequence millions of DNA fragments at once, providing detailed …

Abstract Assay of Transposase Accessible Chromatin sequencing (ATAC-seq) is widely
used in studying chromatin biology, but a comprehensive review of the analysis tools has …

There is a pressing need for allogeneic chimeric antigen receptor (CAR)-immune cell
therapies that are safe, effective and affordable. We conducted a phase 1/2 trial of cord …

Tumor-specific CD8+ T cells (TST) in patients with cancer are dysfunctional and unable to
halt cancer progression. TST dysfunction, also known as exhaustion, is thought to be driven …

Conventional genome engineering with CRISPR–Cas9 creates double-strand breaks
(DSBs) that lead to undesirable byproducts and reduce product purity. Here we report an …

Oestradiol establishes neural sex differences in many vertebrates 1, 2, 3 and modulates
mood, behaviour and energy balance in adulthood 4, 5, 6, 7, 8. In the canonical pathway …

Background Recent innovations in single-cell Assay for Transposase Accessible Chromatin
using sequencing (scATAC-seq) enable profiling of the epigenetic landscape of thousands …

In comparative high-throughput sequencing assays, a fundamental task is the analysis of
count data, such as read counts per gene in RNA-seq, for evidence of systematic changes …

O-GlcNAc is a dynamic post-translational modification (PTM) that regulates protein functions.
In studying the regulatory roles of O-GlcNAc, a major roadblock is the inability to change O …

The function of the CCCTC-binding factor (CTCF) in the organization of the genome has
become an important area of investigation, but the mechanisms by which CTCF dynamically …