Variants of uncertain significance in the era of high-throughput genome sequencing: a lesson from breast and ovary cancers

G Federici, S Soddu - Journal of Experimental & Clinical Cancer Research, 2020 - Springer
The promising expectations about personalized medicine have opened the path to routine
large-scale sequencing and increased the importance of genetic counseling for hereditary …

Genome interpretation using in silico predictors of variant impact

P Katsonis, K Wilhelm, A Williams, O Lichtarge - Human genetics, 2022 - Springer
Estimating the effects of variants found in disease driver genes opens the door to
personalized therapeutic opportunities. Clinical associations and laboratory experiments …

Systematic discovery of protein interaction interfaces using AlphaFold and experimental validation

CY Lee, D Hubrich, JK Varga, C Schäfer… - Molecular Systems …, 2024 - embopress.org
Structural resolution of protein interactions enables mechanistic and functional studies as
well as interpretation of disease variants. However, structural data is still missing for most …

The genetic landscape of the human solute carrier (SLC) transporter superfamily

L Schaller, VM Lauschke - Human genetics, 2019 - Springer
The human solute carrier (SLC) superfamily of transporters is comprised of over 400
membrane-bound proteins, and plays essential roles in a multitude of physiological and …

The clinical imperative for inclusivity: race, ethnicity, and ancestry (REA) in genomics

AB Popejoy, DI Ritter, K Crooks, E Currey… - Human …, 2018 - Wiley Online Library
Abstract The Clinical Genome Resource (ClinGen) Ancestry and Diversity Working Group
highlights the need to develop guidance on race, ethnicity, and ancestry (REA) data …

Multimodal scanning of genetic variants with base and prime editing

O Belli, K Karava, R Farouni, RJ Platt - Nature Biotechnology, 2024 - nature.com
Mutational scanning connects genetic variants to phenotype, enabling the interrogation of
protein functions, interactions and variant pathogenicity. However, current methodologies …

Defining the cell surface cysteinome using two-step enrichment proteomics

T Yan, LM Boatner, L Cui, PJ Tontonoz, KM Backus - JACS Au, 2023 - ACS Publications
The plasma membrane proteome is a rich resource of functionally important and
therapeutically relevant protein targets. Distinguished by high hydrophobicity, heavy …

Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select

Y Niu, CA Ferreira Azevedo, X Li, E Kamali… - Nature Genetics, 2022 - nature.com
Determining the functional role of thousands of genetic sequence variants (mutations)
associated with genetic diseases is a major challenge. Here we present clustered regularly …

Valosin containing protein (VCP): initiator, modifier, and potential drug target for neurodegenerative diseases

S Chu, X Xie, C Payan, U Stochaj - Molecular Neurodegeneration, 2023 - Springer
The AAA+ ATPase valosin containing protein (VCP) is essential for cell and organ
homeostasis, especially in cells of the nervous system. As part of a large network, VCP …

Simple ClinVar: an interactive web server to explore and retrieve gene and disease variants aggregated in ClinVar database

E Pérez-Palma, M Gramm, P Nürnberg… - Nucleic acids …, 2019 - academic.oup.com
Clinical genetic testing has exponentially expanded in recent years, leading to an
overwhelming amount of patient variants with high variability in pathogenicity and …