Selecting and screening recombinant antibody libraries
HR Hoogenboom - Nature biotechnology, 2005 - nature.com
During the past decade several display methods and other library screening techniques
have been developed for isolating monoclonal antibodies (mAbs) from large collections of …
have been developed for isolating monoclonal antibodies (mAbs) from large collections of …
Under the microscope: single-domain antibodies for live-cell imaging and super-resolution microscopy
B Traenkle, U Rothbauer - Frontiers in immunology, 2017 - frontiersin.org
Single-domain antibodies (sdAbs) have substantially expanded the possibilities of
advanced cellular imaging such as live-cell or super-resolution microscopy to visualize …
advanced cellular imaging such as live-cell or super-resolution microscopy to visualize …
In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains
The cilium is an antenna-like organelle that performs numerous cellular functions, including
motility, sensing, and signaling. The base of the cilium contains a selective barrier that …
motility, sensing, and signaling. The base of the cilium contains a selective barrier that …
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower
cost. Here, we describe the first fully synthetic phage display library of humanized llama …
cost. Here, we describe the first fully synthetic phage display library of humanized llama …
Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
Cryofixation has proven to be the gold standard for efficient preservation of native cell
ultrastructure compared to chemical fixation, but this approach is not widely used in …
ultrastructure compared to chemical fixation, but this approach is not widely used in …
X10 expansion microscopy enables 25‐nm resolution on conventional microscopes
S Truckenbrodt, M Maidorn, D Crzan, H Wildhagen… - EMBO …, 2018 - embopress.org
Expansion microscopy is a recently introduced imaging technique that achieves super‐
resolution through physically expanding the specimen by~ 4×, after embedding into a …
resolution through physically expanding the specimen by~ 4×, after embedding into a …
Local palmitoylation cycles define activity-regulated postsynaptic subdomains
Y Fukata, A Dimitrov, G Boncompain… - Journal of Cell …, 2013 - rupress.org
Distinct PSD-95 clusters are primary landmarks of postsynaptic densities (PSDs), which are
specialized membrane regions for synapses. However, the mechanism that defines the …
specialized membrane regions for synapses. However, the mechanism that defines the …
Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis
D Dambournet, M Machicoane, L Chesneau… - Nature cell …, 2011 - nature.com
Abscission is the least understood step of cytokinesis. It consists of the final cut of the
intercellular bridge connecting the sister cells at the end of mitosis, and is thought to involve …
intercellular bridge connecting the sister cells at the end of mitosis, and is thought to involve …
Resolving bundled microtubules using anti-tubulin nanobodies
M Mikhaylova, BMC Cloin, K Finan… - Nature …, 2015 - nature.com
Microtubules are hollow biopolymers of 25-nm diameter and are key constituents of the
cytoskeleton. In neurons, microtubules are organized differently between axons and …
cytoskeleton. In neurons, microtubules are organized differently between axons and …
Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex
S Miserey-Lenkei, G Chalancon, S Bardin… - Nature cell …, 2010 - nature.com
Trafficking between membrane compartments is a characteristic of eukaryotic cells and
relies on transport carriers that bud and fission from a donor membrane, before being …
relies on transport carriers that bud and fission from a donor membrane, before being …