[HTML][HTML] Guidelines for validation of qualitative real-time PCR methods

S Broeders, I Huber, L Grohmann, G Berben… - Trends in Food Science …, 2014 - Elsevier
Highlights•Guidelines for in-house and inter-laboratory validation of qualitative qPCR
methods.•Method acceptance and performance parameters to be evaluated.•Acceptance …

[HTML][HTML] Detecting un-authorized genetically modified organisms (GMOs) and derived materials

A Holst-Jensen, Y Bertheau, M De Loose… - Biotechnology …, 2012 - Elsevier
Genetically modified plants, in the following referred to as genetically modified organisms or
GMOs, have been commercially grown for almost two decades. In 2010 approximately 10 …

Current perspectives on genetically modified crops and detection methods

M Kamle, P Kumar, JK Patra, VK Bajpai - 3 Biotech, 2017 - Springer
Genetically modified (GM) crops are the fastest adopted commodities in the agribiotech
industry. This market penetration should provide a sustainable basis for ensuring food …

Molecular approaches for high throughput detection and quantification of genetically modified crops: a review

IB Salisu, AA Shahid, A Yaqoob, Q Ali… - Frontiers in plant …, 2017 - frontiersin.org
As long as the genetically modified crops are gaining attention globally, their proper
approval and commercialization need accurate and reliable diagnostic methods for the …

[HTML][HTML] Development and applicability of a ready-to-use PCR system for GMO screening

SF Rosa, F Gatto, A Angers-Loustau, M Petrillo… - Food chemistry, 2016 - Elsevier
With the growing number of GMOs introduced to the market, testing laboratories have seen
their workload increase significantly. Ready-to-use multi-target PCR-based detection …

MPIC: a high-throughput analytical method for multiple DNA targets

J Guo, L Yang, L Chen, D Morisset, X Li, L Pan… - Analytical …, 2011 - ACS Publications
We describe the development of a novel combined approach for high-throughput analysis of
multiple DNA targets based on multiplex Microdroplet PCR Implemented Capillary gel …

A novel GMO biosensor for rapid ultrasensitive and simultaneous detection of multiple DNA components in GMO products

L Huang, L Zheng, Y Chen, F Xue, L Cheng… - Biosensors and …, 2015 - Elsevier
Since the introduction of genetically modified organisms (GMOs), there has been on-going
and continuous concern and debates on the commercialization of products derived from …

Loop-mediated isothermal amplification assays: Rapid and efficient diagnostics for genetically modified crops

M Singh, D Pal, P Sood, G Randhawa - Food control, 2019 - Elsevier
With the increase in number of globally approved genetically modified (GM) events and
diversification of traits, efficient GM detection strategies may play key role for regulatory …

Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9 …

F Debode, E Janssen, G Berben - European Food Research and …, 2013 - Springer
Abstract p35S promoter and tNOS terminator are the two primary targets for genetically
modified organism (GMO) screening. An increasing number of genetic constructions do not …

The GMOseek matrix: a decision support tool for optimizing the detection of genetically modified plants

A Block, F Debode, L Grohmann, J Hulin, I Taverniers… - BMC …, 2013 - Springer
Background Since their first commercialization, the diversity of taxa and the genetic
composition of transgene sequences in genetically modified plants (GMOs) are constantly …