Mass spectrometry approaches to glycomic and glycoproteomic analyses

LR Ruhaak, G Xu, Q Li, E Goonatilleke… - Chemical …, 2018 - ACS Publications
Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides
(glycans) conjugated to proteins. Glycans are produced through a complicated nontemplate …

Glycan analysis of therapeutic glycoproteins

L Zhang, S Luo, B Zhang - MAbs, 2016 - Taylor & Francis
Therapeutic monoclonal antibodies (mAbs) are glycoproteins produced by living cell
systems. The glycan moieties attached to the proteins can directly affect protein stability …

Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells

A Croset, L Delafosse, JP Gaudry, C Arod, L Glez… - Journal of …, 2012 - Elsevier
Glycosylation is one of the most common posttranslational modifications of proteins. It has
important roles for protein structure, stability and functions. In vivo the glycostructures …

MS‐based glycomics and glycoproteomics methods enabling isomeric characterization

W Peng, CD Gutierrez Reyes, S Gautam… - Mass spectrometry …, 2023 - Wiley Online Library
Glycosylation is one of the most significant and abundant posttranslational modifications in
mammalian cells. It mediates a wide range of biofunctions, including cell adhesion, cell …

Structural and functional characteristics of bovine milk protein glycosylation

N O'Riordan, M Kane, L Joshi, RM Hickey - Glycobiology, 2014 - academic.oup.com
Most secreted and cell membrane proteins in mammals are glycosylated. Many of these
glycoproteins are also prevalent in milk and play key roles in the biomodulatory properties of …

GPQuest: a spectral library matching algorithm for site-specific assignment of tandem mass spectra to intact N-glycopeptides

S Toghi Eshghi, P Shah, W Yang, X Li… - Analytical …, 2015 - ACS Publications
Glycoprotein changes occur in not only protein abundance but also the occupancy of each
glycosylation site by different glycoforms during biological or pathological processes. Recent …

Glycopeptide enrichment and separation for protein glycosylation analysis

S Ongay, A Boichenko, N Govorukhina… - Journal of separation …, 2012 - Wiley Online Library
Protein glycosylation plays key roles in many biological processes. In addition, alterations in
protein glycosylation have been related to different diseases, as well as may affect the …

The prevalence of sarcopenia in very old individuals according to the European consensus definition: insights from the BELFRAIL study

D Legrand, B Vaes, C Matheï, C Swine… - Age and …, 2013 - academic.oup.com
Background: the prevalence of sarcopenia varies widely between studies. The objective of
this study was to assess the prevalence of sarcopenia in a representative sample of persons …

Caseins: Versatility of their micellar organization in relation to the functional and nutritional properties of milk

A Runthala, M Mbye, M Ayyash, Y Xu, A Kamal-Eldin - Molecules, 2023 - mdpi.com
The milk of mammals is a complex fluid mixture of various proteins, minerals, lipids, and
other micronutrients that play a critical role in providing nutrition and immunity to newborns …

Advances in mass spectrometry‐based glycoproteomics

A Yu, J Zhao, W Peng, A Banazadeh… - …, 2018 - Wiley Online Library
Protein glycosylation, an important PTM, plays an essential role in a wide range of biological
processes such as immune response, intercellular signaling, inflammation, and host …