Expressing cloned genes for protein production, purification, and analysis
CL Kielkopf, W Bauer… - Cold Spring Harbor …, 2021 - cshprotocols.cshlp.org
Obtaining high quantities of a specific protein directly from native sources is often
challenging, particularly when dealing with human proteins. To overcome this obstacle …
challenging, particularly when dealing with human proteins. To overcome this obstacle …
Expression of cloned genes in E. coli using IPTG-inducible promoters
CL Kielkopf, W Bauer… - Cold Spring Harbor …, 2021 - cshprotocols.cshlp.org
Many Escherichia coli expression vectors make use of the lac operon. In general, the lac
operator (lacO) is located downstream from the promoter of the target gene, so that binding …
operator (lacO) is located downstream from the promoter of the target gene, so that binding …
Solubilization of expressed proteins from inclusion bodies
CL Kielkopf, W Bauer… - Cold Spring Harbor …, 2021 - cshprotocols.cshlp.org
The expression of foreign proteins at high levels in Escherichia coli often results in the
formation of cytoplasmic granules or inclusion bodies composed of insoluble aggregates of …
formation of cytoplasmic granules or inclusion bodies composed of insoluble aggregates of …
细菌中蛋白质样品制备方法研究进展.
厉彦鑫, 杨永新, 于忠娜, 王义坚… - Journal of Food …, 2022 - search.ebscohost.com
细菌在生长繁殖时, 细菌蛋白的表达受到环境影响而存在较大差异, 使得细菌蛋白表达具有复杂
性. 在食品生产加工过程中可能会受到致病菌污染, 细菌产生的内毒素和外毒素均会对人体健康 …
性. 在食品生产加工过程中可能会受到致病菌污染, 细菌产生的内毒素和外毒素均会对人体健康 …
High-Throughput Screening of Heterologous Functional Amyloids Using Escherichia coli
Escherichia coli remains one of the most widely used workhorse microorganisms for the
expression of heterologous proteins. The large number of cloning vectors and mutant host …
expression of heterologous proteins. The large number of cloning vectors and mutant host …
Research progress on preparation methods of protein samples in bacteria.
YX Li, YX Yang, ZN Yu, YJ Wang, RW Han, J Wang… - 2022 - cabidigitallibrary.org
The expression of bacterial protein is affected by the environment during growth and
reproduction of bacteria, which makes bacterial protein expression complex. In the process …
reproduction of bacteria, which makes bacterial protein expression complex. In the process …