Next-generation physiology approaches to study microbiome function at single cell level
The function of cells in their native habitat often cannot be reliably predicted from genomic
data or from physiology studies of isolates. Traditional experimental approaches to study the …
data or from physiology studies of isolates. Traditional experimental approaches to study the …
When FLOW-FISH met FACS: Combining multiparametric, dynamic approaches for microbial single-cell research in the total environment
In environmental microbiology, the ability to assess, in a high-throughput way, single-cells
within microbial communities is key to understand their heterogeneity. Fluorescence in situ …
within microbial communities is key to understand their heterogeneity. Fluorescence in situ …
Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions
S Bellais, M Nehlich, M Ania, A Duquenoy, W Mazier… - Microbiome, 2022 - Springer
Background There is a growing interest in using gut commensal bacteria as “next
generation” probiotics. However, this approach is still hampered by the fact that there are …
generation” probiotics. However, this approach is still hampered by the fact that there are …
The oxidoreductase activity of Rnf balances redox cofactors during fermentation of glucose to propionate in Prevotella
B Zhang, C Lingga, H De Groot, TJ Hackmann - Scientific Reports, 2023 - nature.com
Propionate is a microbial metabolite formed in the gastrointestinal tract, and it affects host
physiology as a source of energy and signaling molecule. Despite the importance of …
physiology as a source of energy and signaling molecule. Despite the importance of …
Approaches to investigate selective dietary polysaccharide utilization by human gut microbiota at a functional level
The human diet is temporally and spatially dynamic, and influenced by culture, regional food
systems, socioeconomics, and consumer preference. Such factors result in enormous …
systems, socioeconomics, and consumer preference. Such factors result in enormous …
A new pathway for forming acetate and synthesizing ATP during fermentation in bacteria
B Zhang, C Lingga, C Bowman… - Applied and …, 2021 - Am Soc Microbiol
Many bacteria and other organisms carry out fermentations forming acetate. These
fermentations have broad importance for foods, agriculture, and industry. They also are …
fermentations have broad importance for foods, agriculture, and industry. They also are …
Quantifying fluorescent glycan uptake to elucidate strain-level variability in foraging behaviors of rumen bacteria
Gut microbiomes, such as the microbial community that colonizes the rumen, have vast
catabolic potential and play a vital role in host health and nutrition. By expanding our …
catabolic potential and play a vital role in host health and nutrition. By expanding our …
Technical pipeline for screening microbial communities as a function of substrate specificity through fluorescent labelling
The study of specific glycan uptake and metabolism is an effective tool in aiding with the
continued unravelling of the complexities in the human gut microbiome. To this aim …
continued unravelling of the complexities in the human gut microbiome. To this aim …
Discovering microbiota functions via chemical probe incorporation for targeted sequencing
N Falco, ME Griffin - Current Opinion in Chemical Biology, 2025 - Elsevier
Our microbiota plays crucial roles in immune development and homeostasis and has been
implicated in virtually all major diseases of the 21st century. Nevertheless, our …
implicated in virtually all major diseases of the 21st century. Nevertheless, our …
Recent Methods for the Viability Assessment of Bacterial Pathogens: Advances, Challenges, and Future Perspectives
Viability assessment is a critical step in evaluating bacterial pathogens to determine
infectious risks to public health. Based on three accepted viable criteria (culturability …
infectious risks to public health. Based on three accepted viable criteria (culturability …